Sept. 1,1924 
243 
Bacterial Pustule of Soybean 
Uschinsky’s solution.— Faint to 
moderate clouding. Medium in old 
•cultures (3J^ to 4 months) is the color 
of beef broth. Cultures - of Bad. 
phaseoli sojense sometimes become 
Teddish brown. 
Litmus milk.— Reduction of litmus 
beginning in 4 to 16 days, complete 
an 5 to 19 dsiys (occasionally not com¬ 
plete in 3 weeks). Otherwise as 
recorded in descriptions cited. 
Milk plus methylene blue.— All 
•color disappears in 1 to 2 days. De¬ 
cided yellowish cast to whey and 
ooagulum in 3 weeks. 
Soyka’s rice milk. —Bright yellow 
growth. 
TOLERATION OF ACID AND ALKALI 17 
This work was done in conjunction 
with Quirk and Fawcett in their work 
on “Hydrogen-ion * concentration vs. 
titratable acidity in culture mediums” 
(12, p. 21, SO, 5If). 
Tests of acid and alkali toleration were 
made in 1 per cent peptone beef broth 
(hot infusion A (12,p.21 )),to whichNaOH 
or HCl was added. Growth occurred in 
24 hours in 0 to +17 Fuller’s scale (8.1 
to 6.5 P H ) 17 and in 72 hours Bad. 
phaseoli MRS. had grown in —3 to +28 
Fuller’s scale. (8:4 to 5.7 P H ) 17 , and 
Bad. phaseoli sojense in 0 to +28 (8.1 
to 5.7 Pit). 17 tn 2 weeks both had 
clouded the broth which titrated, at 
the time of inoculation, —6 Fuller’s 
scale (8.7 Ph ), 17 A titration of check 
tubes, however, showed that the 
acidity of this medium had increased 
considerably upon standing. At the 
end of a week the check titrated —3 
Fuller’^ scale (8.3 Ph ) 17 and at the end 
of 2 weeks, at which time growth was 
noted, the check titrated — 1 Fuller’s 
scale (8,2 Ph ). 17 There was still no 
clouding of +32 Fuller’s scale 
(5.238 P H ). 17 A titration of a check 
tube showed that the acidity had 
changed very little on standing 2 
weeks. The check titrated +33 Ful¬ 
ler’s scale (5.2, 5.16 P H ). 17 
TOLERATION OF SODIUM CHLORIDE 
Slight growth in beef bouillon neu¬ 
tral to phenolphthalein plus 4 per cent 
NaCl. Transfers made from the cul¬ 
tures to potato on the thirtieth day 
produced no growth. 
PRODUCTION OF AMMONIA AND 
INDOL 
Ammonia was produced in beef 
bouillon ( + 12 Fuller’s scale, P H 5.8, 
actual determinations) tested on the 
twenty-second day with Nessler’s solu¬ 
tion. No indol was found in Uschinsky 
plus peptone. The sodium nitrite-sui- 
phuric acid test was made on the 
twenty-first day, but there was no pink 
reaction even upon heating. 
VARIETAL DIFFERENCES BETWEEN 
BACT. PHASEOLI SOJENSE AND 
BACT. PHASEOLI EFS. IN PLATE 
CULTURES 
In the preceding tests Bad. phaseoli 
sojense was not distinguishable cul¬ 
turally from Bad. phaseoli EFS. There 
have, however, been some interesting 
differences in the behavior of the colo¬ 
nies on beef-agar plates. 
COLONIES OF BACT. PHASEOLI SOJENSE 
• ON 1 PER CENT BEEF AGAR (MADE 
WITH BEEF INFUSION+ 1 PER CENT 
PEPTONE AND 2 PER CENT AG AR) 
PLATES AT ROOM TEMPERATURE (PL. 
5 AND 6) 
Brief mention was made in a previous 
paper (9) of certain internal markings 
very commonly, though not univer¬ 
sally, appearing in the colonies of Bad. 
phaseoli sojense which serve to dis¬ 
tinguish it in a measure from Bad. 
phaseoli EFS. inasmuch as they have 
been observed but twice in the 
innumerable isolations made from the 
Phaseolus leafspot caused by the 
latter organism. When the colonies of 
Bad. phaseoli sojense first appear (in 
2 or 3 days, rarely 4 or 5) they are 
pale yellow, round, smooth, wet-shining, 
slightly convex, with entire margins 
and no internal markings. At this time 
they are in no way distinguishable from 
Bad. phaseoli EFS. They also become 
a deeper yellow with age like the former, 
but in 5 to 7 days there very commonly 
appear in the surface colonies on the 
more thinly sown plates the aforesaid 
markings which are visible for 1 to 2 
weeks (rarely 3 weeks). They are wholly 
internal, the surface of the colony re¬ 
maining smooth. They are usually 
seen only by oblique transmitted light 
and a hand lens (Zeiss X6) but they 
are sometimes so distinct that they are 
plainly visible by reflected light and 
the naked eye (PI. 5, A). 
There is more than one type of these 
markings, but by far the most common 
and the one which distinguishes Bad. 
phaseoli sojense from Bad. phaseoli 
EFS. is one which the author has 
termed internal convolutions (PI. 5, B). 
There are various modifications of this 
type (PI. 6, A, C, D, F). When they 
17 Potentiometer determinations. 
