Sept. 15,1924 
Infection of Barley by Ustilago Nuda 
283 
of Ustilago nuda produced plants with a 
high percentage of loose-smut. 
Nakano Wase barley remained smut- 
free even .though the seed was dehulled 
and smutted. 
Seedlings from dehulled-inoculated 
seed of all varieties of barley studied 
were severely injured, and many of 
them failed to emerge. 
Seedlings from dehulled-inoculated 
seed sown three-fourths of an inch deep 
emerged better than those from similar 
seed sown inches deep. 
Seedlings from hulled-inoculated seed 
were not noticeably injured. 
Devitalized spores of Ustilago nuda 
were not harmful to barley seedlings 
grown from the inocluated seed. 
Neither viable nor devitalized spores 
ofJJstilago nuda were harmful to seed¬ 
lings of Red Wave wheat grown from 
inoculated seed. 
A miscroscopic study revealed in¬ 
fection of the coleoptile and first leaves 
of the plumule of barley seedlings by 
Ustilago nuda . Both susceptible and 
resistant varieties were infected. 
LITERATURE CITED 
<1) Brefeld, O. 
1888. NEUE UNTERSUCHUNGEN 
UBER DIE BRANDPILZE UND DIE 
BRANDKR ANKHEITEN. ( 11) . 
Nachr. Klub Landw\ Berlin 221: 
1588-1594. 
< 2 ) - 
1903. NEUE UNTERSUCHUNGEN 
UND ERGEBNISSE UBER DIE NA- 
TURLICHE INFEKTION UND VER- 
BREITUNG DER BRANDKR ANKHEI¬ 
TEN des getreides. Nachr. 
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4224-4234. 
(3) -and Falck, R. 
1905. die bluteninfektion bei 
DEN BRANDPILZEN UND DIE NA- 
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Untersuchungen aus dem Ge- 
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1910. VERSUCHE MIT BRAND-IN- 
FEKTION ZUR ERZIELUNG BRAND- 
FREIER GERSTENSTAMME. 
Naturw. Ztschr. Forst. u. 
Landw. 8: 335-344, illus. 
(5) Falck, R. 
1908. DIE FLUGBRANDARTEN DES 
GETREIDES, IHRE VERBREITUNG 
UND BEKAMPFUNG. Jour. 
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(6) Faris, J. A. 
1924. factors influencing in¬ 
fection OF HORDEUM SATIVUM 
by ustilago hordei. Amer. 
Jour. Bot. 11: 189-214, illus. 
(7) Freeman, E. M., and Johnson, 
E. C. 
1909. THE loose smuts of barley 
and wheat. U. S. Dept. Agr. 
Bur. Plant Indus. Bui. 152, 48 
p., illus. 
(8) Hecke, L. 
1904. EIN INNERER KRANKHEITS- 
KEIM DES FLUGBRANDES IM 
getreidekorn. Ztschr. Landw. 
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(9) -- 
1905. ZUR THEORIE DER BLUTEN¬ 
INFEKTION DES GETREIDES 
durch flugbrand. Ber. Deut. 
Bot. Gesell. 23: 248-250, illus. 
(10) Hoffmann, H. 
1866. ueber den flugbrand, us¬ 
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getumpers.). Berlin Bot. Un- 
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(11) Hori, S. 
1907. SEED infection by smut 
FUNGI OF CEREALS. Bui. Imp; 
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163-176. 
(12) Jensen, J. L. 
1888. THE PROPAGATION AND PRE¬ 
VENTION OF SMUT IN OATS AND 
barley. Jour. Roy. Agr. Soc. 
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(13) Johnson, A. G. 
1914. EXPERIMENTS ON THE CON¬ 
TROL OF CERTAIN BARLEY DIS¬ 
EASES. (Abstract) Phytopa¬ 
thology 4: 46. 
(14) Kellerman, W. A., and Swingle, 
W. T. 
1890. REPORT ON THE LOOSE SMUTS 
of cereals. Kans. Agr. Exp. 
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288 
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BEIM WEIZENFLUGBRAND. 
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(16) Maddox, F.. 
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SMUT, BUNT, RUST. 4 p. Dept. 
Agr. Tasmania. 
EXPLANATORY LEGEND FOR PLATE 9 
Infection of barley seedlings by Ustilago nuda. 
Coleoptile infection in sterile tube and dish culture: a, Smyrna (C. 1.195), a two-rowed barley; 6, e , and g. 
Nakano Wase; k and m, Tennessee Winter; o, Han River. 
Coleoptile infection in soil: c, d,f, and i, Han River; j, Tennessee Winter. Infection at base of coleoptile. 
In soil; ?, Han River. 
Infection of first leaf in sterile dish culture: h and n, Han River. 
