Apr. 12, 1924 
Development of American Foulbrood 
147 
/ 
ardized Benedict’s solution are placed in a white porcelain casserole and di¬ 
luted with an equal amount of distilled water. To this are added about 5 grams 
of anhydrous sodium carbonate and a small amount of ground pumice. This 
solution is brought to a boil and the larval extract is run in slowly, drop by 
drop at the end, until the blue color disappears and a white precipitate forms. 
From the number of cc. of larval extract used, the milligrams of sugar per larva 
and the per cent of sugar per larva are calculated (Table V). 
Table V. —Unassimilated sugar in intestinal content of'larvae at different ages 
Larvae of known age, 
Sturtevant ( 86) 
Larvae analyzed for presence of unassimilated sugar (weights in grams) 
Age 
in 
days 
Aver¬ 
age 
weight 
Limits by 
weight 
for age 
groups 
Date 
Weight 
of 
sample 
Num¬ 
ber 
of 
lar¬ 
vae 
Aver¬ 
age, 
weight 
of 1 
larva 
Ex¬ 
tract 
used** 
Equiv¬ 
alent 
number 
of 
larva 
CuS04 
solu¬ 
tion 
Equiv¬ 
alent 
dex¬ 
trose 
Dex¬ 
trose 
per 
larva 
Sugar 
per 
larva 
Gram 
Oram 
1922 
Gram 
Gram 
Cc. 
Cc. 
Gram 
Gram 
P.ct. 
2. 
0.004745 
Up to 
7-18 
0.6233 
50 
0.01247 
50 
50 
5 
No re- 
0 
0 
0.014685. 
action. 
3. 
.024626 
0.014685 to 
7-25 
.4967 
25 
.01987 
50 
25 
5 
No re- 
0 
0 
0.059308. 
action. 
7-25 
1.1072 
25 
b .04429 
44 
22 
5 
0.01020 
0.000463 
1.13 
8-2 
b 1.0979 
25 
*•.04392 
23.5 
23.5 
5 
.01020 
.000434 
.98 
5-9 
*2.2906 
50 
.04581 
90 
45 
10 
.02040 
.000453 
.94 
8-11 
1.1706 
25 
.04682 
42 
21 
5 
.01030 
.000490 
1.04 
7-27 
1.4009 
25 
.05604 
20.25 
10.125 
5 
.01020 
.001007 
1.79 
Average 
.043222 
.000475 
.98 
4. 
0.093990 
0. 059308 to 
8-2 
1. 6749 
25 
.06700 
21 
10.5 
5 
.01020 
~."00097 
1.44 
0.120369. 
8-11 
<* 1.6817 
25 
.06727 
11 
9.16 
5 
.01030 
.00112 
1.66 
8-11 
1.9372 
25 
.07749 
12.75 
6.375 
5 
.01030 
.00161 
2.07 
8-11 
1. 9916 
25 
.07966 
18.1 
9.05 
5 
.01030 
.00113 
1.41 
7-31 
2.3044 
25 
h .09218 
6.8 
3.4 
5 
.01020 
.00300 
3.25 
8-2 
2.3566 
25 
».09426 
8.6 
4.3 
5 
.01020 
.00237 
2.51 
8-30 
2.3733 
25 
.09493 
6.58 
3.29 
5 
.01030 
.00313 
3.29 
7-25 
2.4901 
25 
.09960 
5.8 
2.9 
5 
.01020 
.00351 
3.52 
8-2 
2.6748 
25 
.10699 
8.0 
4.0 
5 
.01020 
.00255 
2.37 
8-17 
2.6843 
25 
.10737 
4.75 
2.38 
5 
.01030 
.00431 
4.01 
8-18 
2. 7781 
25 
.11112 
4.35 
2.175 
5 
.01030 
.00473 
4.25 
8-2 
2. 7919 
25 
.11168 
7.0 
3.5 
5 
.01020 
.00291 
2.61 
8-18 
2.8205 
25 
.11282 
4.35 
2.175 
5 
. 01030 
.00473 
4.19 
6-1 
2.8332 
25 
. 11333 
12.7 
6.35 
8.9 
. 018156 
.00286 
2.52 
8-11 
2.8972 
25 
.11589 
6.8 
3.4 
5 
.01030 
.00303 
2.61 
8-4 
2.9274 
25 
.11710 
7.1 
3.55 
5 
.01020 
.00287 
2.45 
8-2 
2.9505 
25 
. 11802 
7.1 
3. 55 
5 
.01020 
.00287 
2.43 
8-31 
2.9749 
25 
.11900 
5.2 
2.6 
5 
.01030 
.00396 
3.32 
8-2 
2.9908 
25 
.11963 
6.0 
3.0 
5 
.01020 
.00340 
2.84 
8-17 
3.0038 
25 
.12015 
4.5 
2.25 
5 
.01030 
.00457 
3.80 
8-4 
3.0105 
25 
.12042 
6.5 
3.25 
5 
.01020 
.00314 
2.61 
Average 
.10314 
.00299 
2.82 
_ 
_ 
• _ 
_ 
5. 
0.146748 
0.120369 to 
6-1 
c 3.0961 
25 
.12384 
27.0 
6.75 
10 
.02040 
.00317 
2.57 
0.150876. 
8-18 
3.1148 
25 
.12459 
4.2 
2.1 
5 
.01030 
.00490 
3.93 
8-18 
3.1953 
25 
.12781 
4.2 
2.1 
5 
. 01030 
.00490 
3.83 
8-11 
3.2141 
25 
.12856 
6.4 
3.2 
5 
.01030 
.00322 
2.51 
7-26 
3.3153 
25 
.13261 
5.6 
2.8 
5 
.01020 
.00364 
2.75 
7-31 
« 3. 3278 
25 
.13311 
5.0 
2.5 
5 
.01020 
.00408 
3.06 
8—11 
3.3479 
25 
.13392 
6.5 
3.25 
5 
.01030 
.00317 
2.36 
8-10 
3. 3602 
25 
. 13441 
6.15 
3.075 
5 
. 01030 
.00334 
2.45 
7-31 
3.3689 
25 
.13476 
4.1 
2.05 
5 
.01020 
.00497 
3.69 
8-11 
3.3706 
25 
.13482 
6.1 
3.05 
5 
. 01030 
.00337 
2.49 
7-27 
3. 3721 
25 
.13488 
4.5 
2.25 
5 
.01020 
.00453 
3.35 
7-25 
3.4029 
25 
. 13612 
3.4 
1.7 
5 
.01020 
.00600 
4.41 
7-25 
3.4620 
25 
.13848 
5.25 
2.625 
5 
.01020 
.00388 
2.80 
8-17 
3.4644 
25 
.13858 
3.5 
1. 75 
5 
.01030 
.00588 
4.23 
8-31 
3. 4775 
25 
. 13910 
4.6 
2.3 
5 
.01030 
.00448 
3.22 
7-25 
f 3.6156 
25 
.14462 
7.92 
3. 96 
6.07 
.01238 
.00312 
2.15 
8-17 
3.6394 
25 
.14558 
3.6 
1.8 
5 
. 01030 
.00572 
3.92 
8-17 
/ 3. 6971 
25 
.14788 
4.2 
2.1 
5 
. 01030 
.00490 
3.31 
8-18 
/ 3. 7164 
25 
. 14866 
5.2 
2.6 
5 
.01030 
.00396 
2.66 
Average 
_ 1 
. 13591 
.00428 
3.14 
—— 
■ — - 
• - - 
— 
• Unless otherwise stated, total cc. of extract equals 50. 
b Total extract, 25 cc. only. 
e Total extract, 100 cc. 
88286—24-5 
<* Total extract, 30 cc. only. 
« Just sealed, early. 
/ Just sealed, still coiled. 
