148 
Journal of Agricultural Research voi. xxvra, No. a 
Table V.— Unassimilated sugar in intestinal content of larvse at different ages — 
Continued 
Larvae of knownage, 
Sturtevant (85) 
Larvae analyzed for presence of unassimilated sugar (weights in grains) 
Age 
Aver- 
Limits by 
weight 
for age 
groups 
Weight 
Num¬ 
ber 
Aver¬ 
age 
Ex- 
Equiv¬ 
alent 
CuSO* 
Equiv¬ 
alent 
dex¬ 
trose 
Dex¬ 
trose 
Sugar 
in 
age 
Date 
of 
of 
weight 
tract 
number 
solu- 
per 
days 
weight 
sample 
lar¬ 
vae 
of 1 
larva 
used 
of 
larva 
tion 
per 
larva 
larva 
Gram 
Gram 
ms 
Grams 
Gram 
cc. 
cc. 
Gram 
Gram 
P.ct. 
6. 
0.155005 
0.150876 to 
7-18 
«3.8012 
25 
0.15205 
18.05 
4.51 
9.6 
0.19584 
0.00434 
2.85 
maximum 
8-31 
3.8038 
25 
. 15215 
5.4 
2.7 
5 
. 01030 
.00381 
2.50 
and down 
8-11 
3.8925 
25 
.15570 
4.35 
2.175 
5 
. 01030 
.00473 
3.03 
to 0.148326. 
8-31 
* 3.9783 
25 
. 15913 
6.0 
3.0 
5 
. 01030 
.00343 
2.15 
8-31 
4.1249 
25 
.16500 
7.0 
3.5 
5 
.01030 
.00294 
1.78 
7-27 
3.7706 
25 
.15082 
6.65 
3.33 
5 
.01020 
.00306 
2.03 
Average 
.15581 
.00372 
2.39 
7. 
0.141048 
0.148326 to 
8-18 
3.6980 
25 
. 14792 
7.3 
3.65 
5 
.01030 
.00282 
1.91 
0.139406. 
8-10 
3.5600 
25 
.14280 
8.9 
4.45 
5 
.01030 
.00231 
1.56 
8-10 
h 3.5835 
25 
.14334 
11.5 
5.75 
5 
. 01030 
.00179 
1.25 
8-4 
3. 5572 
25 
.14229 
7.4 
3.7 
5 
.01030 
.00275 
1.93 
8-18 
i 3.4871 
25 
.13948 
45.0 
22.5 
5 
.91030 
.00050 
.36 
Average 
.14397 
.00203 
1.40 
8. 
0.137165 
0.139406 to 
7-26 
3.4358 
25 
.13743 
50.0 
25 
5 
No re- 
0 
0 
0.135158. 
7-18 
i 3.4453 
25 
.13781 
50.0 
25 
5 
action. 
No re¬ 
action. 
0 
0 
Average 
. 13762 
0 
0 
9. 
0.133152 
8-4 
*3.3232 
25 
.13293 
50.0 
25 
5 
No re- 
o 
0 
action. 
e Total extract, 100 cc. 
* All sealed, coiled or with backs out. Feeding ended and spinning of cocoons started. 
* Cocoon partially spun, still some color in the intestine. 
i Cocoon not quite finished, still moving somewhat, no color in intestine. 
i Quiescent prepupae, intestines colorless, empty, histolysis started. 
* First indication of change in external form. 
OBSERVATIONS 
Over 60 samples of 25 larvse each of various ages, containing over 1,600 indi¬ 
vidual larvse, were analyzed for the presence of reducing sugars. The largest 
number of analyses were made^on larvse from 3? to 5| days of age during the 
active honey and pollen feeding period. At least five analyses were made of 
each of the other age periods which might show the presence of sugar. To obtain 
averages with a small probable error, the analyses are grouped by age periods of 
24 hours each, as described earlier (Table V, fig. 13). All larvse in the two- 
day group, as well as one sample of larvse nearly as heavy as the three-day aver¬ 
age larva, showed no reducing sugar. Larvse in the three-day group, averaging 
0.043222 gm. in weight, gave 0.000475 gm. of reducing sugar per larva, or 
0.98 per cent concentration. Larvse in the four-day group, averaging 0.10314 
gm. in weight, gave 0.00299 gm. of reducing sugar per larva, or 2.82 per cent 
concentration. Larvse in the five-day group, comprising those just prior to 
sealing, with a few just sealed, averaging 0.13591 gm. in weight, gave 0.00428 
gm. of reducing sugar per larva, or 3.14 per cent concentration. In the five-day 
group there were two samples which gave a concentration of over 4 per cent, the 
maximum being 4.41 per cent. The six-day group, comprised entirely of larvse 
that had been sealed, had finished feeding and had started spinning, averaging 
0.15581 gm. in weight, gave 0.00372 gm. of reducing sugar per larva, or 2.39 per 
cent concentration. This group contains larvse of maximum size (fig. 14). From 
