158 
Journal of Agricultural Research 
Vol. XXVIII, No. 2 
the percentage concentration in the intestine, at least during the progressive feed¬ 
ing period, should be approximately five times as great as the value calculated (in 
Table V) on the basis of the entire larval weight. The calculated percentages 
for the third and fourth days are now 4.90 and 14.10, respectively, and on the 
fifth day, just before sealing, the sugar concentration in the intestine should ap¬ 
proximate 15.70 per cent (fig. 16). There is, of course, the factor of dilution, 
APPROX. P&&OP OP* %Pf¥?OX PPR/OD "APPROX. PFWOO *APPPOX/MAr£P&VCH> CXPf£7X- 
Fig. 16.—Correlation of time of death from American foulbrood to per cent concentration of reducing sugar 
in entire larva and to calculated per cent concentration in the intestine. Per cent sugar in the food and 
per cent glycogen in the larva are shown for comparison 
particularly toward the end of the feeding period, caused by the accumulation of 
undigested pollen shells, which may lower this figure somewhat. 
Still another approximate check may be calculated from the molecular weights of 
dextrose, glycogen, and fat, and from the percentage composition of the constitu¬ 
ents of the larva (Tables IV and V), in order to obtain the percentage of sugar 
which was present in the intestine at any one time previous to assimilation neces- 
