174 
Journal of Agricultural Research 
Vol XXVIII, No. 2 
PREPARATION OF EXTRACTS OF COTTONSEED KERNELS 
The cottonseed kernels were extracted in a Soxhlet extraction apparatus until 
no significant yellow color was imparted to the ether which bathed the thimble 
and contents, a procedure that required from 18 to 48 hours. The ether was 
evaporated from the extract with the aid of heat, and the residual oil, diluted with 
peanut oil, was used for the experiments in the manner described. 
DIETS 
In th£ feeding tests four diets were used: Control peanut meal; gossypol; raw 
cottonseed kernel; and control ether-extracted cottonseed kernel. The first 
three contained an abundance of peanut meal, an adequate source of protein. 
All diets contained an abundance of butterfat as a source of fat-soluble vitamin, 
an abundance of milk powder supplying protein, and an adequate salt mixture. 
They contained roughage in the form of agar-agar, except those containing the 
greatest quantity of each variety of raw cottonseed kernels, which were used 
only for a few of the shorter feeding experiments. 
The control peanut meal diet and the gossypol diet differed only in the presence 
of gossypol. The control ether-extracted cottonseed kernel diet was the same 
as the other diets, except that it contained ether-extracted cottonseed kernels 
and additional fat instead of peanut meal or whole cottonseed kernels. The 
gossypol and fat had been removed from the cottonseed kernels by ether extrac¬ 
tion in the course of quantitative gossypol analyses formerly made ( 9 ). The 
raw cottonseed kernel diet was the same as the control diet except that part of 
the peanut meal and fat had been replaced by different varieties of raw cotton¬ 
seed kernels in varying proportions. The composition of the various kernels 
and diets is given in Tables II and III. In each case the diet contains all the 
ingredients essential for normal growth. 
Table I .—Ether extracts injected intraperitoneally 
Variety extracted 
Cottonseed 
Ether extract 
Gossypol 
in diluted 
extract for 
injection 
Quantity 
extracted 
Gossypol 
content 
Gossypol 
content 
Peanut oil 
added to 
make— 
Trice__-__ 
Qm. 
54.6 
42.2 
26.2 
19.2 
Per cent 
0.411 
.518 
.984 
1.180 
Mgm. 
224 
218 
257 
227 
Cc. 
50 
50 
50 
50 
Per cent 
0.440 
.437 
.514 
.454 
Lone Star.... 
Durango..... 
Egyptian.. .. 
Table II .—Composition of cottonseed kernels and other material 
* Material 
Ether 
extract 
Nitrogen 
Moisture 
Gossypol 
Trice kernels . _ 
Per cent 
28. 87 
33.40 
38.97 
36. 68 
60 
60 
(<0 
10. 33 
Per cent 
6.42 
6.40 
4.94 
4.73 
9.01 
8.59 
8.30 
8.11 
Per cent 
6.12 
Per cent 
0.416 
.518 
.984 
1.180 
60 
60 
0 
0 
Lone Star kernels. _ ._ _ 
Durango kernels _. _ _ 
5.93 
5.66 
10.92 
Egyptian kernels__ 
Ether-extracted cottonseed kernels I®.. 
Ether-extracted cottonseed kernels II ®._... 
Peanut meal I _ __ 
Peanut meal II_ _ 
6.59 
a Composite samples resulting from the chemical analyses reported by Schwartze and Alsberg ( 9 ). 
& Gossypol and fat were removed by ether extraction. A trace of each was probably present, 
c Only a few experiments were made with this sample. Ether extract was assumed to be 10.33 per cent in 
making the calculations. 
