Apr 12, 1924 
Toxicity of Cottonseed and Its Gossypol Content 183 
four male rats averaged 207 gm., being 64 and 52 gm., respectively, below the 
average weight attained on the two control diets and 31 gm. below the average of 
the male rats fed on a diet containing 0.0675 per cent of gossypol. The average 
weight of the two female rats fed 0.09 per cent gossypol was below the average 
weight of the female rats on the other diets by 58, 38, and 35 gm., respectively. 
Fig. 9.—Effect of 0.135 per cent gossypol in the diet. u C” indicates change to control peanut meal diet 
Only a few experiments were made with a concentration of 0.135 per cent of 
gossypol in the diet. The results, such as they are, indicate that the retardation 
of growth was marked and the resumption of growth poor. There is no question 
but that the poor resumption of growth is to be regarded as an effect of the 
gossypol, for the sturdiness of stock from which these rats came and the adequacy 
of the diet were certain. 
Eig. 10.—Effect of diet containing cottonseed kernels estimated to contain approximately 0.0675 per cent 
crude gossypol. “C” indicates change to control peanut meal diet 
DIET CONTAINING ADDED RAW COTTONSEED KERNELS 
The result of individual experiments in which raw cottonseed kernels were 
added to the diet in such proportions that the diet as a whole contained approxi¬ 
mately 0.0675 and 0.135 per cent of gossypol are given in figures 10 and II. 
Owing to the comparatively small number of experiments with each variety of 
kernel, separate average curves for each variety are not given. Instead, a com¬ 
posite curve for each sex for all kernels, based upon the average body weight of 
all the rats of each sex fed approximately the same estimated concentration of 
