464 Joumal of Agricultural Research y 0 i. xxvm, No. s 
Hens' Eggs 
The eggs used in the tests for vitamin B were laid by hens kept under similar 
conditions and fed the same feeds as those that were killed. The eggs were 
collected in September, 1922,'when the hens were receiving a considerable quan¬ 
tity of green feed. At that time the hens were nearing the end of their laying 
season. 
METHOD OF DRYING POULTRY FLESH AND EGGS 
The flesh, fat, and skin of the frying chickens were ground together, but the 
muscle tissue of the hens and other birds was separated from the skin and from 
the fat, as far as practicable, before grinding. The ground flesh was thoroughly 
mixed with water and toluol in the proportion of 800 grams, 400 cubic centi¬ 
meters, and 40 cubic centimeters, respectively, and the mass was spread out in 
shallow pans and dried in an oven in a current of air at a temperature that did 
not exceed 60° C. The tissue became dry in 18 to 20 hours, after which it was 
ground, transferred to bottles, and held in cold storage until needed. 
The eggs were separated from the shells, thoroughly beaten, spread out in a 
thin layer in shallow pans, and dried in a current of air at a temperature of 60° C. 
FEEDING TESTS WITH RATIONS VERY DEFICIENT IN ANTINEURITIC 
VITAMIN 
In order to judge the antineuritic value of a ration containing the product to 
be tested, it is necessary to have for comparison the results of other experiments 
conducted in the same manner in which the pigeons have been fed rations practi¬ 
cally devoid of antineuritic vitamin. In Table I are reported- the results of a 
number of such tests previously reported by one of the writers (2, 3). 
Table I .—Feeding tests with rations very deficient in antineuritic vitamin 
Rations 
Ingredients 
Percent 
Pigeon 
No. 
Initial 
weight 
Final 
weight 
Change 
in weight 
Survival 
period 
Result 
Pen 1: 
Grams 
Grams 
Per cent 
Days 
Autoclaved rice. 
100 
2,061 
366 
348 
—4.9 
18 
Polyneuritis. 
2,062 
362 
284 
-21.5 
18 
Do. 
2,064 
316 
255 
-19.3 
28 
Do. 
2,065 
425 
303 
-28.7 
25 
Do. 
Average. 
367 
298 
-18.6 
22 
Pen 2: 
Autoclaved rice. 
100 
249 
425 
383 
-9.9 
17 
Polyneuritis. 
250 
470 
273 
-41.9 
46 
Do. 
251 
389 
282 
-27.5 
24 
Do. 
252 
419 
306 
-27.0 
24 
Do. 
Average. 
426 
311 
-26.6 
28 
Pen 3: 
Autoclaved rice_.. 
76 
| 2,066 
305 
280 
-8.2 
21 
Polyneuritis. 
Autoclaved beef_ 
15 
1 2,067 
362 
320 
-11.6 
21 
Do. 
Cod-liver oil. 
5 
| 2,068 
304 
256 
-15.8 
27 
Do. 
Ash mixture.-. 
4 
J 2,069 
300 
225 
-25.0 
29 
Do. 
Average. 
318 
270 
-15.2 
25 
Pen 4: 
Autoclaved rice- 
76 
1 245 
379 
329 
-13.2 
21 
Polyneuritis. 
Autoclaved beef. 
15 
I 246 
396 
275 
-30.6 
42 
Do. 
Butterfat.. 
5 
f 247 
377 
304 
-19.4 
28 
Do. 
Ash mixture. 
4 
j 248 
335 
311 
-7.2 
21 
Do. 
Average. 
372 
305 
-17.6 
28 
