482 
Journal of Agricultural Research 
Vol. XXVIII, No. 5 
Table II.— Effect of dilution on the color value of glucose solutions 
Glucose 
solution 
Final 
volume 
Colori¬ 
meter 
reading 
Recovery 
Per cent 
Centimeter 
Millimeter 
Per cent 
0.08 
10.0 
30.0 
100 
0.16 
20.0 
28.5 
105 
0.32 
40.0 
27.7 
108 
0.64 
80.0 
27.4 
110 
Table III .—Comparison of clarification methods 
Sucrose 
Reducing sugar 
Total sugar 
Material 
Not 
clarified 
Clarified 
Not 
clarified 
Clarified 
Not 
clarified 
Clarified 
Per cent 
Per cent 
Per cent 
Per cent 
Per cent 
Per cent 
LEAD ACETATE 
Corn-stalk juice___ 
5.50 
5.53 
3.15 
3.28 
8.65 
8.81 
Do..... 
10.23 
10.06 
2.93 
2.97 
13.16 
13.03 
Do...__ 
5.14 
5.11 
2.96 
3.04 
8.10 
8.14 
Do_____ 
8.82 
8 . 92 
3.23 
3.02 
12.15 
11.94 
Do_____ 
6.62 
6.70 
2.93 
2 . 97 
9.55 
9.67 
Do.... 
11.42 
11.26 
3.03 
3.04 
14.45 
14. 30 
Apple juice.... 
8.40 
8.46 
Grape juice____ 
17.80 
17.66 
% 
ALCOHOL 
Corn-stalk juice___-.. 
5.43 
5.42 
3. 36 
3. 37 
8.79 
8.76 
Do____ 
4.54 
4. 56 
3.20 
2.98 
7.74 
7.54 
SODIUM TUNGSTATE 
Corn-stalk syrup ... 
14.50 
11.10 
18.10 
14.50 
14.56 
11.30 
18.10 
14.40 
Sorghum syrup .. . _ _. 
Do 
* 
Diastophore 
PICRIC ACID 
Sorghum syrup..... 
37.26 
39.33 
34. 92 
32.04 
72.18 
71. 37 
Do____ 
24. 25 
27. 67 
35.64 
33.12 
59.89 
60.79 
Table III shows the results obtained by various methods of clarification. 
When lead acetate was used deleading was accomplished with potassium oxalate. 
Alcohol clarification of corn juice was obtained by heating 400 cc. of juice 
with 600 cc. of 95 per cent alcohol for 20 minutes in a water bath. The alcoholic 
filtrate was diluted 25 times with water and 1 cc. was used for a sample, without 
removing the alcohol. 
Rumsey’s directions (17) for clarification with sodium tungstate were followed, 
an acidity of approximately P H 2 being obtained with sulphuric acid. The 
filtrate was diluted 100 times and used for analysis without the removal of the 
excess tungstate. The “diastophore” is a malt extract preparation. 
