tn ^ 
492 
Journal of Agricultural Research 
Vol. XXVIII, No. 5 
No. Treatment 
1 _Liquid pure culture applied as usual. * * 3 
2 _No treatment. 
3 _Liquid pure culture applied in excess and allowed to stand 
one hour before spreading out to dry. 
Dry soil applied by the glue method. 4 
Dry soil applied by the glue method dried 10 days previous to 
planting. 
6 _Liquid pure culture applied as usual, dried 10 days before 
planting. 
7 _Dry soil applied by sowing in rows with seed. 
8 _Liquid pure culture applied as usual dried 24 hours before 
planting. 
9 _No treatment. 
10 _Liquid pure culture applied as usual. 
Fig. 1 —Chart showing percentage of observed wilt disease symptoms among navy beans under the dif¬ 
ferent treatments. Entirely black bars represent the moisture treated rows 
The same plan of experiment was conducted at the Great Plains stations of 
the Office of Dry Land Agriculture at Mandan, N. Dak.; Akron, Colo.; Wood¬ 
ward, Okla.; and at the station of the Office of Forage Crop Investigations, 
Redfield, S. Dak. It was conducted under irrigated soil conditions at the farm 
of the Office of Foreign Seed and Plant Introduction at Chico, Calif., and under 
humid conditions at the Arlington Experiment Farm of the United States De¬ 
partment of Agriculture at Rosslyn, Va., and on private ground at Leelancb 
Montgomery County, Md. Conditions at these places are indicated in Table II. 
Liquid pure culture was applied at the approximate rate of 10 cc. per 500 gm. of bean seed, mixed with 
the seed by hand and at once spread out to dry. The excess application was made similarly with double 
the quantity of liquid, with the exception that the seed were covered with paper one hour before being 
s pread out to dry. 
4 a 5 per cent glue solution is prepared by adding furniture glue to boiling water and cooling. The seed 
are moistened with this mixture and dry infected soil sifted over them to absorb the moisture and adhere 
to the seed coats. 
