582 
Journal of Agricultural Research voi. xxviii, no. & 
means have shown that the substance in this extract that causes the skin reaction 
is absent from the globulin fraction. 
(5) It is present in the albumen fraction, and is also present in the filtrate after 
precipitation of the albumen and globulin fractions by ammonium sulphate. 
(6) It is weakened by oxidation with potassium permanganate. 
(7) It is not volatile at temperatures between 20° and 100° C. 
(8) It is thermolabile and is destroyed by exposure to a temperature of about 
100° C. for less than an hour, but may survive exposure to a temperature as high 
as 100°C. acting for a period of about 15 minutes. 
(9) It is destroyed in the albumen fraction by digestion with pepsin. 
(10) It is soluble in 50 per cent alcohol. 
(11) From the filtrate obtained after precipitation of the globulin and albumen 
fractions with ammonium sulphate it is wholly adsorbed in the presence of acid 
by Lloyd’s reagent, and wholly precipitated by Mayer’s solution. 
(12) It is also wholly adsorbed from the acidified albumen fraction by Lloyd's 
reagent but has not been recovered from the latter by subsequent treatment with 
weak alkali, a procedure which releases it from Lloyd’s reagent after adsorption 
from the aqueous extract from which the albumen and globulin fractions have 
been removed by precipitation with ammonium sulphate. 
(13) The question whether the substance in Ascaris lumbricoides that causes 
the skin reaction in sensitive human subjects is of protein nature has not been 
answered by the investigations herein reported. 
(14) A substance or substances that cause local reactions in some hogs and 
some sheep, comparable to the reactions observed in human subjects, are present 
in Ascaris lumbricoides. The ophthalmic reactions observed in these animals are 
altogether similar to those heretofore observed by Weinberg and Julien following 
the instillation of the body-cavity fluid of Ascaris equorum into the eyes of horses. 
LITERATURE CITED 
(1) Flury, F. 
1912. ZUR CHEMIE UND TOXIKOLOGIE DER ASCARIDEN. Arch. Exp. Path, 
u. Pharmakol. 67: 275-392. 
(2) Goldschmidt, R. 
1910. die askarisvergiftung. Munchen. Med. Wchnschr. 57: 1991— 
1993. 
(3) Ransom, B. H., and Foster, W. D. 
1920. OBSERVATIONS ON THE LIFE HISTORY OF ASCARIS LUMBRICOIDES. 
U. S. Dept. Agr. Bui. 817, 47 p., illus. 
(4) - 
1922. OBSERVATIONS ON THE TOXIC EFFECTS OF ASCARIS FLUIDS. Jour. 
Parasitol. 9: 42-43. 
(5) Shimamura, T., and Fujii, H. 
1917. UEBER DAS ASKARON, EINEN TOXISCHEN BESTANDTEIL DER HEL- 
MINTHEN BESONDERS DER ASKARIDEN UND SEINE BIOLOGISCHE WIRKUNG. 
(mitteilung I). Jour. Col. Agr. Imp. Univ. Tokyo 3: 189-258, illus. 
(6) Weinberg, M., and Julien, A. 
1911. SUBSTANCES TOXIQUES DE L’ASCARIS MEGALOCEPHALA. RECHERCHES 
exp^rimentales sur le cheval. Compt. Rend. Soc. Biol. [Paris] 70: 
337-339. 
(7) - 
1911. exemple d’immunite acquise vis-a-vis d’une toxine vermineuse. 
Compt. Rend. Acad. Sci. [Paris] 152: 1030-1032. 
1913. recherches sur la toxine ascaridienne. Hyg. Viande et Lait 
7: 225-244, illus. 
(8) 
