Journal of Agricultural Research voi. xxvii, No. a 
104 
not in agreement with the results of 32 days' exposure to 39 0 F., as 
shown in Table III). Eleven days' exposure to 32 0 or to 20° F. gave 
a complete kill, judging from the conditions found in the samples, while 
18 days' exposure to 39 0 F. did not kill the infestation. 
Table IV. —Results of dissection of refrigerated cowpeas 
:n removal 
on and dis- 
1 
m 
T 3 
Stages dissected from cowpeas. 
0 
1 
<v 
Number of cowpeas 
sected. 
Adults. 
i 
Pupae. 
Larvae. 
I 
f 
! 
Temperature. 
•5 
0 
c 
.2 
-M 
2 
3 
P 
Interval bet wee 
from refrigerati 
section. 
1 Alive. 1 
1 ! 
, 
p 
Emerged before 
dissection. ! 
Alive. 1 
Dead. 
i 
Alive. 
j 
Dead. 
Total. 
Dead. 
°F. 
20. 
Days. 
4 
Days . 
IO 
50 
3 
7 
O 
7 
8 
9 
27 
61 
Per cent. 
69 
20.. 
11 
4 
13 
0 
6 
O 
2 
5 
1 
I • 
I b 
80 
20. 
11 
Months. 
7 
37 
0 
8 
O 
0 
10 
O 
IOO 
118 
IOO 
20. 
18 
7 
50 
0 
17 
O 
0 
33 
O 
106 
156 
IOO 
20. 
25 
7 
50 
0 
20 
0 
0 
40 
O 
-89 
149 
IOO 
20.. 
32 
6 
100 
0 
34 
O 
0 
82 
0 
184 
300 
IOO 
32 .. 
11 
6 
5 ° 
0 
15 
O 
0 
29 
O 
87 
13 1 
IOO 
32 ,. 
18 
6 
50 
0 
19 
O 
0 
52 
0 
94 
i 6 5 
IOO 
32 .- • • 
25 
6 
50 
0 
23 
O 
0 
65 
O 
72 
160 
ICO 
32 . 
32 
6 
100 
0 
28 
O 
0 
83 
O 
236 
347 
IOO 
39 . 
11 
6 
50 
0 
12 
8 
0 
37 
O 
97 
154 
95 
3.9 . 
18 
6 
50 
0 
22 
4 
0 
42 
0 
88 
97 
39 -. 
25 
6 
5 ° 
0 
21 
0 
0 
47 
O 
80 
148 
IOO 
39 .. 
; 32 
i 
6 
100 
0 
18 
0 
0 
70 
O 
261 
349 
IOO 
Control. 
4 
100 
3 
9 
48 
93 
17 
11 
121 
302 
49 
EFFECT OF REFRIGERATION ON THE VIABILITY OF THE EGGS 
Table V shows that four days’ refrigeration at 32 0 or 20° F. is suffi¬ 
cient to prevent all eggs from hatching; a temperature of 39 0 F. for 
the same length of time, however, leaves some eggs viable. Of the eggs 
removed from a temperature of 39 0 F. after four days’ refrigeration 
and exposed to favorable temperatures, three hatched and one of the 
three larvae developed and emerged. 
Table. V .—Effect of refrigeration on the viability of eggs of Bruchus quadrimaculatus 
; 
j Duration of 
| refrigeration. 
1 
i 
Tem¬ 
pera¬ 
ture. 
j 
Num¬ 
ber of 
beans 
re¬ 
moved. 
! 
Num¬ 
ber of 
eggs. 
Num¬ 
ber 
hatch¬ 
ed. 
Duration of 
refrigeration. 
Tem¬ 
pera¬ 
ture. 
Num¬ 
ber of 
beans 
re¬ 
moved. 
Num¬ 
ber of 
eggs. 
Num¬ 
ber 
hatch¬ 
ed. 
| Days. 
1 4 . 
| 11. 
18. 
1 25. 
i J 
| 32 . 
! 4 . 
i 11 . 
! 18. 
1 
°F. 
20 
20 
20 
20 
20 
32 
32 i 
32 | 
1 
5 o 
50 
50 
50 
IOO 
50 
1 5 ° 
1 50 
1 
a 
a 
a 
a 
664 
a 
a 
a 
0 
O 
O 
O 
O 
0 
0 
0 
Days. 
25 . 
32 . 
4 . 
11. 
18. 
1 
25 . 
32 . 
Control. . . 
°F. 
32 
32 
39 
39 
39 
39 
39 
50 
IOO 
50 
50 
• 50 
50 
IOO 
IOO 
a 
656 
a 
a 
a 
a 
697 
662 
j 
O 
O 
h 3 
0 
0 
0 
0 
456 
a Not counted. 
b Produced i adult. 
