386 
Journal of Agricultural Research voi. xxvii. no. « 
To determine the relative number of stomata on the leaf, seedlings of 
wheat varieties were grown in the greenhouse. When the seedlings were 
io days old, the first leaf of several plants of each variety was cut off 
near the base and placed in aceto-alcohol until clear. The entire leaf was 
then mounted in aceto-alcohol and observed through strong transmitted 
light. Counts were made both on the midrib and at the outside of the 
leaf, i inch and 2 inches from the tip. 
Table III.—Average number of stomata in 10 fields, each containing 2.138 sq. mm., 
from both surfaces on different parts of the leaves of five wheat varieties 
Variety. 
Khapli, C. I. 4013: 
Lower surface. 
Upper surface. 
Kanred, C. I. 5146: 
Lower surface. 
Upper surface. 
Marquis, C. I. 3641: 
Lower surface. 
Upper surface. 
Little Club, C. I. 4066: 
Lower surface. 
Upper surface. 
Kota, C. I. 5878: 
Lower surface. 
Upper surface. 
On midrib. 
Edge of blade 
Average 
for 
leaf. 
Distance from tip. 
Distance from tip. 
1 inch. 
2 inches. 
1 inch. 
2 inches. 
56 
57 
52 
49 
54 
69 
80 
78 
87 
78 
37 
47 
42 
45 
43 
70 
78 
71 
81 
75 
i 52 
34 
45 
28 
40 
! 79 
78 
92 
84 
83 
44 
50 
44 
38 
44 
| 70 
i 
81 
73 
89 
79 
36 
35 
32 
33 
34 
56 
55 
59 
1 
56 
56 
both 
surfaces. 
66 
64 
61 
61 
45 
TabIvB IV. Size of stomatal apertures on seedling leaves of different wheat varieties in 
the greenhouse , at different times in a winter day with bright sunlight from Q.45 a. m. 
t° 4-35 P- m -> at University Farm, St. Paul, Minn. 
Variety and data on stomatal 
Time of day. 
openings. 
9 -is 
n.15 
I-I 5 
1 
3. IS 
5-15 
Little Club, C. I. 4066: 
Aperture in micra... 
32-1x0.5 
34.3x4.35 
40.3 x 7.4 
38.9 X 7.8 
Closed. 
Percentage open. . 
1 
32 
IOO 
IOO 
0 
Khapli, C. I. 4013: 
Aperture in micra... 
Percentage open.... 
22.6 x 0.5 
1 
22.6 x 0.5 
60 
22.6 X O. 5 
75 
24.2 X 0.5 
20 
22.6 x 0.5 
c 
Kanred, C. I. 5146: 
Aperture in micra... 
34.2x0.5 
35-9 x 5.1 
39.4X 6.3 
40.5 X 5.0 
Closed. 
Percentage open.... 
60 
95 
95 
95 
0 
Mindum, C. I. 5296: 
Aperture in micra... 
Percentage open.... 
23-5x0.5 
40 
28.1 x 1.0 
80 
31.3x0.9 
10 
26.1 x 0.7 
10 
31-3x0.5 
e 
Marquis, C. I. 3641: 
D 
Aperture in micra... 
Closed. 
33 -ox 1.3 
33.0 x 1.3 
Closed. 
Closed. 
Percentage open.... 
0 
1 
1 
0 
0 
Kota, C. I. 5878: 
Aperture in micra... 
35-0x0.5 
36.9 x 2.1 
35.1X 2.9 
35.1x 3.1 
Closed. 
Percentage open.... 
20 
20 
70 
1 
0 
