Feb. 16, 1924 
Flag Smut of Wheat 
481 
were used, but Flemming’s triple stain and Heidenhain’s iron-haema- 
toxylin used alone or with orange G in clove oil counterstain gave the 
most satisfactory results. 
Owing to the thickness of the epispore and surrounding envelope of 
sterile cells, the first divisions of the nucleus within the spore were not 
observed. The young pro mycelium, however, contained a variable 
number of nuclei, derived from the single nucleus of the spore (fig. 2, 
