Eeb. 23, 1924 
Utilization of Lactose by the Chicken 
601 
These tests indicated that for the purpose of this investigation either 
method could be used with a fair degree of accuracy. Both methods 
were employed throughout and the technique used was the same as that 
recommended for each method when applied to urine analysis. In order 
to obtain additional data and also to serve as a check on the quantitative 
results, the phenyl-hydrazine test was uniformly applied to all extracts. 
The osazones were examined microscopically only. 
Tabi,E III.—‘Summary of results on the utilization of lactose by the hen 
Experi¬ 
ment 
No. 
Daily diet. 
I 
Basal ration plus 6 gm. lactose 
II 
Basal ration plus 2 gm. lactose 
III 
Basal ration. 
IV 
Basal ration plus 18 gm. lac¬ 
tose. 
V 
Basal ration. 
VI 
Basal ration plus 24 gm. lac¬ 
tose. 
VII 
Basal ration. 
1 
Dura¬ 
tion of 
Presence of osa¬ 
zones in excreta. 
Total reducing sub¬ 
stances excreted 
calculated as lac¬ 
tose, CijHmOii. 
experi¬ 
ment. 
Lactos- 
azone. 
Glucos- 
azone. 
Munson- 
Walker- 
Bertrand 
method. 
Benedict 
method. 
1 
Days. 
Gm. 
I. 98 
Gm. 
I. 70 
2 
7 
—• 
— 
O. 80 
O. 64 
3 
— 
I. 21 
O. 78 
4 
— 
— 
i- 57 
I. 84 
1 
— 
— 
0. 00 
O. 16 
2 
1 
— 
— 
0. 00 
O.O3 
3 
— 
— 
0. 00 
O.03 
4 
— 
— 
0. 00 
O. 15 
1 
— 
— 
4-34 
i- 34 
2 
7 
— 
— 
3 - 29 
1. 40 
3 
— 
— 
0.63 
0. 60 
4 
— 
— 
1. 54 
0. 72 
1 
_ 
+ 
5 - 52 
3 - 89 
2 
7 
— 
+ 
6.08 
3 - 78 
3 
+ 
+ 
5. 16 
4. OS 
4 
. 
+ 
+ 
10. 84 
7 . 78 
1 
— 
+ 
3.08 
O. 56 
2 
7 
— 
+ 
2. 03 
i- 33 
3 
— 
+ 
3 - 7 i 
1.68 
4 
— 
+ 
2. 52 
1 
+ 
+ 
3.08 
1. 79 
2 
•7 
+ 
! + 
8. 16 
5 - 3 6 
3 
+ 
j + 
6. 11 
3 - 52 
4 
. 
+ 
! + 
9-37 
7. 07 
1 
_ 
i + 
2.38 
0. 70 
2 
7 
— 
i + 
3. 01 
1. 26 
3 
— 
+ 
2. 73 
0. 98 
4 
— 
! + 
3 - i 5 
1. 61 
Hen 
No. 
DISCUSSION 
The basal ration consisted of 30 gm. of a mixture of oats and cracked 
corn, morning and evening, and 30 gm. of moistened mash at noon. 
The mash consisted of equal parts by weight of wheat bran, flour mid- 
dlings, ground com, ground oats, and tankage. The lactose fed dunng all 
the experiments was Merck's U. S. P. lactose monohydrate which proved 
