7io 
Journal of Agricultural Research 
Vol. XXVII, No. 9 
serum alone was completely inactive. It was found that about 0.15 
cc. of fresh pigeon serum hemolyzed 1 cc. of a 5 per cent suspension of 
rabbit corpuscles in the presence of 0.2 cc. of inactivated chicken serum. 
Ludke ( 4 ) discusses hemagglutinins in chicken and duck serum for 
the red blood corpuscles of other species. He found that fresh serum 
from a 3 day-old chick agglutinated and dissolved human and rabbit 
blood corpuscles in about 10 minutes, but this serum lost the lytic power 
and most of the agglutinating power after being heated for one-half hour 
at 55° C. 
Serum from a normal 3-day-old duck agglutinated blood corpuscles of 
the sheep almost immediately, in dilutions of 1 part to 10, and slight!}' 
in dilutions of 1 part to 20. The hemagglutinins disappeared when the 
serum was heated to 6o° to 65° C. Fresh duck serum caused complete 
agglutination of guinea-pig corpuscles in 5 minutes. Such serum show r ed 
a trace of agglutination for human corpuscles and a strong agglutination 
of rabbit corpuscles followed by lysis. After heating this serum at 
50° to 55 0 C. there was partial agglutination of guinea-pig cells, none for 
human corpuscles and complete agglutination in 5 minutes for those of 
the rabbit. After heating at 55 0 to 6o° C. no agglutination for guinea 
pig or human corpuscles was observed, but complete agglutination for 
rabbit corpuscles occurred in one-half hour. 
Rywosch (7) studied the hemolytic action of the blood of embryo 
chicks upon rabbit corpuscles. No hemolysis could be demonstrated 
with embryo chick serum. One-tenth cc. of serum from a chick 5 days 
of age caused complete hemolysis of 0.2 cc. of 5 per cent rabbit cell sus¬ 
pension and was almost complete for 0.5 cc. This author found that 0.08 
cc. of serum from an adult bird caused complete hemolysis of 1 cq. of 5 
per cent suspension of rabbit corpuscles. An attempt to show that the 
negative results were due to lack of amboceptor failed. These tests 
were conducted with varying amounts of chick embryo serum and inacti¬ 
vated serum from adult birds. Serum from 5-day-old chicks added to the 
inactivated serum from adult birds caused complete hemolysis. This 
author seemed to think that the embryo serum lacked both amboceptor 
and complement for rabbit cells. 
Sherman (8) examined blood from chick embryos and reported as 
follows: In no case was lysin found in any embryo except those of 21 
days’ incubation, and in this case the chicks were pecking their way out of 
the shell. Their serum contained lysin for rabbit erythrocytes only, o. 1 
cc. of serum being required to lake completely 0.1 cc. of a 1 per cent 
erythrocyte suspension. Complement was found in the embryo serums 
of 17 and 21 days’ incubation; it required 0.05 cc. of the serum of the 
younger embryo and o. 1 cc. of that of the older to lake completely o. 1 
cc. of 1 per cent suspension of rabbit erythrocytes wfien 1 unit of ambo¬ 
ceptor (dog) was added. No embryo serum examined contained com¬ 
plement for sheep, goat, or human erythrocytes. 
Rissling (6) studied the hemolytic and hemagglutinating action of the 
serum of various species upon the blood corpuscles of others. He used 
fresh blood corpuscles. These were washed twice and used in 1 per cent 
suspension in salt solution. For agglutination tests the serum was 
inactivated at 56° C. for one-half hour. The tests were incubated at 
37 0 C. for two hours. Goose serum was found to agglutinate sheep cells 
when diluted to 1 part to 10. Duck and chicken serum in these dilutions 
did not agglutinate sheep cells. Chicken serum caused agglutination of 
corpuscles of various animals in the following dilutions: Human, 1 to 50; 
