Journal of Agricultural Research 
Vol. XXVII, No. 
7H 
An examination of Table IV shows that, under the conditions of the 
test, there is very little natural antisheep hemolysin in chicken serum 
that is capable of being activated by guinea-pig complement. No 
hemolysis occured in any tube containing less than i cc. of diluted 
complement, and the degree of hemolysis in these tubes appeared to be 
the same in all cases. A preliminary titration showed that i cc. of a 
i to io dilution of this pooled guinea-pig serum would not cause hemo¬ 
lysis of 0.5 cc. of a 5 per cent suspension of sheep cells. 
Assuming the possibility that the natural antisheep hemolysin might 
be better activated by the native complement in chicken serum, it was 
decided to make such a test on each sample as indicated in Table IV. 
The tubes were thoroughly shaken and placed in the incubator at 
37 0 C. for one hour and placed in the refrigerator overnight. Table V 
gives the results obtained. 
Table IV .—The hemolytic action of fresh unheated chicken serum on sheep cells 
Tube. | 1 
2 
3 
4 
5 
Serum (undiluted). 0. 1 
0. 2 
o *3 
0. 4 J 
! 0.. 
Cells (5 per cent) . .5 
■ 5 1 
• 5 
• 5 i 
• 5 
Saline.; 1. 4 
1. 7. | 
1. 2 
1. 1 i 
\ 1.0 
j 
0 \ 
i 
1 
Table V. —The hemolytic and hemagglutinating action of fresh unheated chicken serum 
on sheep cells 
.. ( 
0.5 cc. of sheep cells plus serum. 
1 . 
Smallest 
1 
[ 
quantity 
1 
1 
of serum 
j 1-20 
1-10 
[ 1-6.6 
1-5 
i 1-4 
which 
Bird No. 
i 
! 
caused 
t 
agglutina¬ 
! 
1 
tion of 
0.1 cc. 
0.2 cc. 
i 0.3 CC. 
1 
0.4 CC. 
1 0.5 cc. | 
i ; 
blood cells. 
1 
1 
| 
1 Cc. 
1. 
a o 
° 
0 
i 0 
1 + 
O. 4 
2 . 
0 
! ° 
+ 
+ 
+ + 
. 1 
3 . 
0 
! 0 
i 4 - 
+ 
! Not run. 
. 2 
4 . 
0 
j 4 - 
! «++ 
++ 
! ++ 
. 2 
5 . 
0 
0 
0 
++ 
1 ++ 
. 2 
6 . 
0 
0 
I 0 
+ 
0 
• 3 
7 . 
0 
0 
; 0 
0 
0 
• 5 
8 . 
0 
1 « + + + 
! + + + 
+ + + : 
1 +++ 
. 1 
9. 
0 
+ 
I ++ 
++ i 
++ 
. 2 
10. 
0 
! + 
! + + 
++ i 
. 2 
11. 
0 
O 
+ 
++ 
++ 
. 2 
12.1 
O i 
+ 
1 + 
T+ ! 
++ 
. 2 
17 . 
O 
O 
0 
+ ! 
. 2 
14. 
O 
O 
0 
+ 
++ 
. 1 
15. 
° ! 
O 
' + 
T 
•3 
16. 
° i 
O 
+ + 
+ -f 
++ 
. 2 
17. j 
0 ; 
O 
-f 
4 " 
++ 
. 2 
18. i 
0 
O 
+ 
++ 
++ 
•3 
19.; 
0 
0 
+ 
++ 
. 2 
20 .! 
0 ; 
O 
0 
0 
4 - 
b . 2 
21 . 
0 i 
0 
0 
+ 
+ 4 - 
. 2 
22 . 
0 ; 
+ + 
T + 
. 2 
a + + +-= marked; + +=partial; +=trace; 0= no hemolysis. 6 Very slight. 
