Mar. i, 1924 
Antisheep Amboceptor and Complement in Fowls 715 
In all sera tested some agglutination of the red cells took place. The 
smallest quantity of serum causing agglutination is indicated in Table V. 
It will be seen that some hemolysis occurred in every case except with 
the serum of fowl No. 7. Variation in the degree of hemolysis is evident; 
in general the complement titer corresponds in a measure to the 
hemolytic titer of the fresh serum. The hemagglutinin does not appear 
to correspond in strength to the complement titer. Nor do the hemag- 
glutins correspond in strength to the hemolytic action of the fresh serum. 
CONCLUSIONS 
Most samples of fresh chicken serum are capable of causing some 
hemolysis of sheep cells. The small quantity of natural antisheep 
amboceptor contained in normal chicken serum may be separated from 
the complement by absorption with sheep cells at o° C. The quantity 
of hemolysin does not vary appreciably in different individuals. The 
quantity of complement is small as compared with that contained in 
guinea-pig serum. A variation in the quantity of complement existed 
in the samples tested. Hemagglutinins for sheep cells are present in 
fowl sera but the quantity is not in proportion to the degree of hemolysis. 
LITERATURE CITED 
(1) Aschenheim, E. 
1909. UEBER DIE NATURUCHEN HAMOEYTISCHEN ZWISCHENKORPER DES 
menschuchEN beutes. Centbl. Bakt. (I, Orig.) 49: 124-138, illus. 
(2) Bordet, J. 
1899. AGGLUTINATION ET DISSOLUTION DES GEOBUEES ROUGES PAR EE S^RUM. 
Ann. Inst. Pasteur 13: 273-207. 
(3) Hyde, R. R. 
1921. A STUDY OF THE NATURAE AND ACQUIRED ANTISHEEP HEMOLYSINS OF 
RABBIT AS REGARDS THERMOEABILITY. Amer. Jour. Hyg. 1: 346-357. 
(4) LudkE, H. 
1906. beitrage zur kenntnis der hamaggeutinine. Centbl. Bakt. (I, 
Orig.) 42: 69-74,' 150-155, 255-265. 
(5) Muller, P. 
1901. ueber antihamoeysine. Centbl. Bakt. (I, Orig.) 29: 175-187. 
(6) Risseing, P. 
1907. beitrage zur bioeogie normaeer tiersera. Centbl. Bakt. (I, Orig.) 
44: 669-683. 
(7) Rywosch, M. 
1907. UEBER HAMOEYSE UND BAKTERCIDIE DES EMBRYONALEN HUHNER' 
beutes. Centbl. Bakt. (I, Orig.) 44: 468-474. 
(8) Sherman, H. W. 
1919. antibodies in the chick. Jour. Infect. Diseases 25: 256-258. 
additional copies 
OF THIS PUBLICATION MAY BE PROCURED FROM 
THE SUPERINTENDENT OF DOCUMENTS 
GOVERNMENT PRINTING OFFICE 
WASHINGTON, D. C. 
AT 
IO CENTS PER COPY 
Subscription Price, $4.00 Per Year (Domestic) 
$5.25 Per Year (Foreign) 
V 
