236 
Journal of Agricultural Research 
Vol. XXVI, No. 5 
Experiments 2 and 4 indicate that death was not due to a lack of 
dissolved oxygen in the water. 
The following experiments were made in duplicate and identical re¬ 
sults were obtained in each case. 
5. One-tenth gm. of gossypol, 25 cc. H 2 0 2 and 20 gm. of ether-extracted, 
unheated cottonseed meal were mixed and added to 5 liters of water in 
which two fish had been placed. The fish remained normal for nine 
hours. 
6. One-tenth gm. of gossypol and 25 cc. of H 2 0 2 were added to 5 liters 
of water and two fish were placed in the solution. The fish died in if 
hours, as in experiments 3 and 4. 
7. One-tenth gm. of gossypol, 25 cc. H 2 0 2 and 20 gm. of ether-extracted, 
“hot-pressed” cottonseed meal were mixed and added to 5 liters of 
water, and two fish were dropped into the liquid. The fish died in if 
hours, as in experiments 3, 4, and 6. 
Gossypol is toxic to fish as a dilution of 1:100,000; hydrogen peroxid 
does not destroy its toxicity when in solution. Hydrogen peroxid, in 
conjunction with unheated cottonseed meal, destroys the toxicity of 
gossypol when in solution, probably through the agency of a peroxidase 
enzym. 
ANALYSIS OF THE BLOOD AND URINE OF ADULT SHEEP ON A DIET 
OF COTTONSEED MEAL 
An adult male sheep was fed 1 pound of cottonseed meal per day, 
beginning April 10. The sheep was kept on green pasture except during 
the days when it was confined in a metabolism cage for the collection of 
the urine. Samples of blood and urine were collected at intervals, and 
upon analysis gave the results shown in the following table. The system 
of blood analysis by Folin and Wu (5) was followed for the determination 
of the blood constituents, and the methods outlined in Hawk’s Practical 
Physiological Chemistry ( 4 ) were used for the analysis of the urine. 
BLOOD CONSTITUENTS 
Apr. 
4 * 
Apr. 
11. 
May 
9. 
May 
18. 
May 
25 - 
June 
2. 
June 
8. 
June 
i 5 - 
June 
24. 
June 
30. 
Non-protein N in mgm. per ioo 
cc. t blood. 
Sugar (per cent). 
32-4 
• 069 
33 
.071 
46.02 
.087 
44-43 
.084 
45 
.086 
44 
.087 
4 i -3 
.088 
3 i -4 
.064 
30 
.049 
28.8 
.047 
URINE CONSTITUENTS 
Apr. 
16. 
May 
2. 
May 
i 6. 
May 
15. 
May 
21. 
May 
24. 
May 
31- 
June 
3 - 
June 
20. 
June 
28. 
Volume in cc. 
Specific gravity. 
Total N in gm. 
Urea N in gm. 
Ammonia N in gm. 
Creatinin N in gm. 
Total acetone bodies in gm. 
500 
1.026 
5-92 
3-506 
1-33 
1-2 
•25 
1*250 
1.032 
19.09 
12.24 
1-34 
1. 21 
1. 2 
1,910 
1.026 
22.22 
16.39 
1.05 
1.24 
2,350 
1.02 
23-25 
17-36 
1.071 
1.07 
1,600 
1.017 
28.0 
2 
2, IOO 
1.023 
23-85 
16.93 
2.99 
I. 2 
1,260 
1-033 
22.38 
17-14 
2.04 
i -5 
3 
1,800 
1.030 
30-0 
24.12 
2.07 
1 - 3 
2- I 
1,550 
1.0325 
26.26 
19.05 
3-33 
1-3 
1-5 
1,600 
1.026 
30.76 
16.66 
i -77 
1.4 
1-7 
Cottonseed meal is here shown to have a diuretic action. At first the 
concentration of the nonprotein nitrogen and sugar of the blood are 
increased, but after the second month they are lowered far below the 
