240 
Journal of Agricultural Research 
Vol. XXVI, No. 5 
jar, and at once taken to the laboratory, where it was weighed. The 
iron content was determined on air-dry material. The loss in drying 
was noted, and the iron found was calculated to parts of FejOg per thous¬ 
and parts of fresh sample. The iron was determined on i-gm. samples 
by the iodometric method of A. Neumann 3 after destruction of the 
organic matter by digestion with a mixture of sulphuric acid and nitric 
acid. Care was necessarily taken to use reagents that were free from 
iron, and samples were at no time exposed to contamination by metallic 
iron or its salts. The total red counts were made in the usual manner, 
the ordinary precautions being observed. The blood was procured from 
the under surface of the tail. The hemoglobin was determined by the 
Talquist method. 
Table I. — Iron content of horses' spleen in infectious equine anemia 
No. 
Description of animal. 
Date. 
Spleen 
weight. 
Solids, 
aiivdry 
parts per 
1 , 000 . 
FesOa 
fresh 
parts per 
x.ooo. 
Total 
FetOs. 
Gm. 
Gm, 
17 
Young, normal; shot. 
Mar. 2 
910 
220. 6 
0. 29 
0. 26 
9 
Aged, normal; shot. 
Mar. 18 
1, 240 
238. 6 
5 - 59 
6.93 
2 
Aged, normal; shot. 
May 27 
1,025 
253 - 3 - 
4-23 
4-34 
22 
Young, bled liters, Aug. 8; 
shot. 
Aug. 12 
787 
244. 0 
. 48 
-39 
20 
Young, bled 10 liters, Aug. 8; 
shot. 
Aug. 14 
910 
234 . 5 
. 28 
•25 
6 
Young, acute infectious equine 
anemia; died. 
Apr. 16 
6, 257 
250.0 
1. 18 
7 - 38 
21 
Aged, acute infectious equine 
anemia; died. 
Sept. 29 
5 ,233 
260. 0 
1. 08 
5 - 65 
26 
Aged, acute infectious equine 
anemia; died. 
Feb. 23 
5 . “9 
271.4 
1 .45 
7. 42 
753 
Young, chronic infectious 
equine anemia; shot. 
Feb. 26 
1,025 
228. 9 
•43 
•44 
23 
Aged, chronic infectious 
equine anemia; shot. 
Feb. 25 
i ,934 
222.0 
2. 48 
4. 80 
18 
Aged, chronic infectious 
equine anemia; shot. 
Feb. 10 
1.36s 
236.1 
1.58 
2.15 
25 
Aged, chronic infectious 
equine anemia; shot. 
Mar. 18 
1.365 
222. 3 
4.44 
6. 06 
Averages: 
Acute infectious equine 
anemia. 
5 , 536 
260.4 
1. 23 
6. 81 
Bled. 
848. 5 
239. 2 
•38 
•32 
'M’OFtrial , . 
1,038 
237-5 
3 - 37 
3 - 84 
Chronic infectious equine 
anemia . 
1,422. 2 
227. 5 
2. 23 
1.91 
"Normal aged. 
1,132. 5 
245 - 9 
4.91 
5 * 6 3 
"Normal yon ng. 
910 
220. 6 
. 29 
. 26 
Total agod . 
2,468. 7 
243. 5 
2. 98 
5 - 33 
Total young. 
1 . 997-8 
* 3 S- 6 
•53 
1. 74 
As has been previously stated, this study is based on a very small 
number of cases and, therefore, any statements made must be guarded. 
However, the results of these determinations on the blood and spleens 
* Neumann, Albert, ueber bine einfachb methods der eisenbbstimmung bei stoffwbchsbl- 
versuchen. a. Mittheilung. In Arch. Anat. u. Physiol., Physiol. Abt., 190a, p. 363-365* I 9 ° a - 
