Dec. 8,1923 
Dourine 
501 
TEST FOR GEOBUUN 
The Ross-Jones * test for globulin was applied to the spinal fluids, of 
horse 128 and blackleg calves 1, 2, and 3. The test consists in layering 
1 cc. of spinal fluid on 2.0 cc. of a saturated solution of ammonium 
sulphate. A white or gray ring at the point of contact of the two fluids 
indicates a positive reaction. The spinal fluid from horse 128 gave a 
5 COLOftLESS 
4 pale: blue: 
3 BLUE* 
2 PUPPLE 
/ /TED BLUE 
O PED 
Fig. 3.—Colloidal gold test of spinal fluid of blackleg calf No. 2. 
markedly positive reaction, while the fluid from calves 1,2, and 3 gave a 
clear, cut negative reaction. 
CELI/ COUNT 
For the cell count the ordinary white corpuscle pipette and blood¬ 
counting chamber were used. The diluting fluid consisted of 0.3 per 
S COLOPLESS 
4 PALE BLUE 
3 BLUE 
2 PURPLE 
/ RED BLUE 
/\ 
O PED 
/ 
V 
O 
O 
O 
/ 
0 
0 
0 
0 
0 
a 
Fig. 4. —Colloidal gold test of spinal fluid of blackleg calf No. 3 - 
cent glacial acetic acid in distilled water. An average of three counts of 
fluid from horse 128 showed 180 cells per cubic millimeter. In the three 
fluids from the blackleg calves no cells were observed. As the cells in 
normal fluids may vary from o to 10, the count of 180 in the fluid of 
• Ross, George W., and Jones, Ernest, on the use of certain new chemical tests in the diagnosis 
OF GENERAL PARALYSIS AND TABES. In Brit. Med. Jour., 1909 , V. I, p. IIII-III 3 . 1909 - 
