5io 
Journal of Agricultural Research voi. xxvi, no. x© 
On 2 per cent glycerin agar: In every particular resembled very 
closely growth on commeal agar. 
Effect of temperatures on growth: On commeal growth was more 
rapid at 25 0 C. than at 7 0 C. but at the latter temperature the surface of 
the media (10 cc. in a 100 cc. Erlenmeyer flask) was covered with growth 
and conidial production was abundant in one week; germination was 
prompt at 7 0 C. 
On August 27, 1920, twigs were taken from healthy peach trees and the 
leaves were removed to expose the newly formed buds. Part of these 
twigs were then sprayed with water containing conidia from pure cul¬ 
tures. The inoculated twigs were placed under a bell jar and others 
sprayed with sterile water were placed under a separate bell jar and 
regarded as checks or controls. In 10 days the fungus had invaded the 
buds, leaf scars and the cut upper end of the inoculated twigs forming 
white sporodochia on their surfaces. The leaf and blossom buds of the 
inoculated twigs were killed, whereas those of the checks remained 
healthy and after 26 days came out into leaf. The fungus was reisolated 
from the killed buds. Using the methods outlined above, inoculations 
were made after the leaves had fallen naturally on October 12, and on 
November 19, 1920, also on February 7, March 1, March 11, buds showing 
pink, and March 16, blossoms out, 1921. In all cases the inoculations 
were successful, and eventually the fungus could be made to fruit on the 
killed buds by placing them under conditions of sufficient moisture. In 
each experiment the fungus was reisolated from the inoculated buds. 
The buds or blossoms subjected to inoculation and those used as con¬ 
trols were cut open and examined with the following results: 
Experiment of Oct. 12, 62.5 percent of buds on inoculated peach twigs were dead,, 
control buds all alive. 
Experiment of Nov. 19, 53 per cent of buds on inoculated peach twigs were dead, 
control buds all alive. 
Experiment of Feb. 7, 98 per cent of buds on inoculated peach twigs were dead, 
control buds all alive. 
Experiment of Mar. 1, 49 per cent of buds on inoculated peach twigs were dead, 
3 per cent of control buds were dead. 
Experiment of Mar. n, 75 per cent of buds on inoculated peach twigs were dead, 
9 per cent of control buds were dead. 
Experiment of Mar. 16, 100 per cent of blossoms on inoculated peach twigs were 
dead, control blossoms all alive. 
On February 19, Elberta and Champion nursery peach trees, growing 
in pots in the greenhouse, were sprayed with a suspension of conidia in 
water; bell jars were placed over the trees for four days. The half- 
opened blossoms were killed, as were most of the flower buds, and the 
fungus was fruiting on their surfaces. 
Inoculations of both sweet and sour cherry blossom-buds were made 
on November 19 and March 11. Twigs were removed from the trees, 
sprayed with a suspension of spores in water and placed under bell jars. 
Other twigs sprayed with sterile water only were placed under bell jars 
and regarded as checks. The buds used on March 11 were much 
swollen, showing green at the tips. The results after one week were as 
follows: 
Sweet Cherry: 
Nov. 19, 100 j>er cent of buds on inoculated twigs were dead. Control buds 
were all alive. 
Mar. 11, 72 per cent of buds on inoculated twigs were dead; 9% of the control 
buds were dead. 
