Mar. 1,1925 
Vaccination Against Rabies in Dogs 
433 
additional dogs each received one in¬ 
jection, making a total of 18 vaccinated 
dogs. Nine of these were given a vac¬ 
cine made in this laboratory (desig¬ 
nated B. A. I.) and 9 were vaccinated 
with vaccines prepared by three com¬ 
mercial firms (designated respectively 
A, B, and C.). On September 12, 
1922, the 18 vaccinated dogs together 
with 8 control dogs received an injec¬ 
tion of 0.1 c. c. of rabies street virus 
in the anterior chamber of the left 
eye. The virus consisted of a 1:10 
dilution in salt solution of the hippo¬ 
campus and medulla of a dog dead of 
rabies. 
The rabies street virus used in ex¬ 
periment No. 1 was obtained from a 
dog which developed the disease spon¬ 
taneously at the Bureau of* Animal 
Industry Experiment Station, Be- 
thesda, Md. This dog, No. 474, was 
purchased from the District of Col¬ 
umbia pound on August 22, 1922, and 
was being held for use as a control or 
check dog in a rabies experiment. On 
September 10 the animal developed 
clinical symptoms of furious rabies and 
was found dead September 11. The 
diagnosis of rabies was confirmed by 
microscopic examination and subse¬ 
quently by rabbit inoculations. On 
September 12 a 1:10 dilution of the 
hippocampus and medulla of this dog 
was made in salt solution and used as 
the exposure virus in experiment No. 1. 
This virus was designated Bureau of 
Animal Industry No. 474. 
The data of experiment No. 1 are 
given in Table I. 
Table I .—Data of experiment No. 1 
Dog 
No. 
Vac- . 
cine * 
Amount 
injected 
Date vaccine 
was injected 
Date exposed 
to virus b 
Result • 
Date of rabbit 
inoculation 
Result 
C. c. 
1 
A_ 
5 
May 25,1922 
Sept. 12,1922 
Dead Nov. 2, 
Nov. 2,1922 
Dead, Nov. 16, 
1922; rabies. 
1922; rabies. 
2 
A. 
5 
.do_ 
_do. 
Dead, Sept. 30, 
Sept. 30,1922 
Dead, Oct. 14, 
1922; rabies. 
1922; rabies. 
3 
B. 
5 
_do. 
_do... 
Dead, Sept. 29, 
.do.. 
Dead Oct. 15, 
1922; rabies. 
1922; rabies. 
4 
B. A. I. 
5 
_do. 
.do. 
Dead, Oct. 2, 
Oct. 3,1922 
Do. 
1922; rabies. 
5 
B. A. I. 
5 
.do. 
.do. 
Alive, Mar. 7, 
1923. 
6 
B. A. I. 
5 
.do. 
i 
t 
< 
( 
b- 
o 
1 
1 
1 
1 
1 
1 
1 
Dead, Sept. 29, 
Sept. 30,1922 
Do. 
1922; rabies. 
7 
B. 
5 
_do. 
.do. 
Alive, Mar. 7, 
1923. 
8 
A. 
5 
July 18,1922 
.do. 
Dead, Sept, 24, 
Sept. 27,1922 
Dead, Oct. 17, 
1922; rabies. 
1922; rabies. 
9 
B. A. I. 
3 
_do. 
_do. 
Dead, Sept. 26, 
.do. 
Dead, Oct. 9, 
1922; rabies. 
1922; rabies. 
10 
B. A. I. 
5 
.do. 
.do.. 
Alive, Mar. 7, 
1923. 
11 
B. A. I. 
5 
.do.. 
_do. 
Dead, Sept. 28, 
Sept. 30,1922 
Dead, Oct. 15, 
1922; rabies. 
1922; rabies. ' 
12 
B. A. I, 
3 
.do. 
.do. 
Dead, Sept. 25, 
Sept. 28,1922 
Dead, Oct. 9, 
1922; rabies. 
1922; rabies. 
13 
C. 
5 
_do.. 
.do. 
Dead, Sept. 24, 
Sept. 27,1922 
Dead, Oct. 11, 
1922; rabies. 
1922; rabies. 
14 
B. A. I. 
5 
.do. 
.do. 
.do... 
Sept. 26,1922 
Dead, Oct. 9, 
1922; rabies. 
15 
B. A. I. 
5 
_do_ 
_do_ 
_ do. __ 
Dec. 7,1922 
Dead, Dec. 21, 
1922; rabies. 
16 
B. 
5 
_do_... 
.do. 
.. do. 
Sept. 26,1922 
Dead, Oct. 6, 
1922; rabies. 
17 
B. 
5 
_do_ 
.do. 
Dead, Sept. 25, 
Sept. 28,1922 
Dead, Oct. 17, 
1922; rabies. 
1922; rabies. 
18 
B . 
3 
.do. 
-do. 
Dead, Sept. 28, 
.do. 
Dead, Oct. 11, 
1922; rabies. 
1922; rabies. 
19 
Control 
_do_ 
Dead, Oct. 24, 
Oct. 24,1922 
Alive, Dec. 24, 
dog. 
1922; enteritis. 
1922. 
20 
...do_ 
_do_ 
Alive, Jan. 16, 
1923. 
21 
—do.... 
_do. 
.do.. 
22 
...do.— 
_do_ 
. do _ 
23 
—do_ 
_do__ 
_do_ 
24 
—do—. 
_do_ 
_do_ 
25 
...do_ 
.do. 
_do_ 
26 
...do_ 
_do.... 
Dead, Oct. 9, 
1922; rabies. 
° Vaccines A, B, and C were from three different commercial houses manufacturing this product. 
Vaccine B. A. I. was prepared in the laboratory of the Bureau of Animal Industry. 
b The virus consisted of a 1:10 dilution in salt solution of the hippocampus and medulla of dog 474, 
dead of rabies; 0.1 c. c. of this material was injected into the anterior chamber of the left eye. 
« The diagnosis of rabies was made by microscopic examination, and in the case of vaccinated dogs by 
rabbit inoculations. The surviving control dogs were destroyed Jan. 16, 1923, and the surviving vac¬ 
cinated dogs were destroyed Mar. 7, 1923. 
19974—25f-4 
