Mar. l, 1925 
1 ’acdnation Against Rabies in Dogs 
439 
In this event the extent to which the 
single-injection vaccine will assist in 
the control and eradication of rabies in 
dogs will depend in a large measure on 
the extent and distribution of the 
strain of virus designated B. A. I. 474 
or similar strains, until such time as 
polyvalent vaccines can be prepared 
from more than the one strain of fixed 
virus as is now the case. 
There is a possibility that the injec¬ 
tion of a vaccine prepared according to 
the method of Umeno and Doi may re¬ 
sult in death from fixed-virus infection, 
as seen in the case of dog 440. 
The subcutaneous injection of fixed 
virus, even without attenuation, is 
generally considered to be harmless. 
This is undoubtedly true in general, 
but occasionally an individual may be 
encountered that is extremely sus¬ 
ceptible and infection may result, even 
with an attenuated fixed virus. The 
percentage of such individuals, how¬ 
ever, is believed to be small. 
Umeno and Doi state that of the 
31,307 vaccinated dogs there was one 
case of vaccination loss. It is pre¬ 
sumed from this statement that the 
loss was due to fixed-virus infection 
following vaccination. 
SUMMARY 
The foregoing work may be sum¬ 
marized as follows: 
The virus to which vaccinated dogs 
were exposed was the factor which 
determined the efficacy of the single¬ 
injection method of vaccination. 
Against one virus no protection was 
afforded vaccinated animals. 
[.^Against two other viruses distinct 
protection was afforded. 
The vaccines prepared in this coun¬ 
try by commercial houses are all made 
from a strain of fixed virus having its 
origin in the Pasteur Institute of Paris, 
France. 
There appears to be more than one 
strain of rabies street virus in this 
country. 
