Apr. 1,1925 
685 
Investigations of Infectious Equine Anemia 
No. 2684.—On March 6, healthy 
gelding 8 years of age, weighing 1,015 
lbs., was given, partly subcutanously 
and partly intravenously, 350 c. c. of 
a solution made by taking 250 c. c. of 
whole clotted blood from No. 2561, a 
typical field case, and shaking it with 
physiological salt solution and strain¬ 
ing out the clumps of fibrin. The 
first symptoms were noted on March 
18 when the animal appeared dejected 
and listless. He gradually grew worse 
and on April 6 there was edema of the 
abdomen and prepuce, and the mucous 
membranes of the eyes and nose were 
reddish yellow. On April 11 the 
weight was 910 lbs. On April 15 he 
was very weak, dejected, and passed 
bloody urine. Death occurred on 
April 16. The hemoglobin and red 
weakness, loss of flesh and spirits, 
edema of the prepuce and pale yellow¬ 
ish mucous membranes—were noticed 
in about six weeks. This condition 
gradually became worse with develop¬ 
ment of edema of the legs and abdo¬ 
men, nasal hemorrhage and polyuria. 
The blood drawn from the veins was 
thin, did not clot readily, and the 
corpuscular volume was about one- 
fifth the total volume. The serum 
was tinged with yellow. The general 
condition of the animal gradually im¬ 
proved and he was in fairly good form 
during the latter part of October. 
Some severe exercise at this time, how¬ 
ever, was apparently too much for his 
weakened resistance; he rapidly be¬ 
came worse and died on November 4. 
At the time of death the hemoglobin 
Fig. 2—Temperature record of animal No. 2563 
cells gradually decreased and the 
lymphocytes slowly increased during 
the illness of the animal (fig. 3). 
No. 2932.—On August 25, aged 
healthy gelding, weighing 1,195 lbs., 
was given 50 c. c. of whole blood, 
intravenously, from experimental case 
No. 2796. Oq September 12, 200 
c. c., and on September 25, 400 c. c. 
more were given. The first symptoms 
with edema were noticed on October 6. 
The animal gradually became worse 
with loss of flesh and spirits and con¬ 
tinued ill until early in January, when 
he apparently recovered and remained 
well until he was killed on February 
17, being apparently healthy at this 
time. On October 9 the hemoglobin 
was 60 per cent, the red cells 5,834,000 
and the lymphocytes 42.2 per cent. 
This condition gradually improved 
and was normal at the death of the 
animal (fig. 4). 
No. 2283.—On August 21, a middle- 
aged healthy gelding weighing 1,070 
lbs., was given intravenously 30 c. c. 
of defibrinated blood from a typical 
subacute case. The first symptoms— 
Fig. 3.—Temperature record of animal No. 2684 
was 40 per cent and the red cells 
3,936,000 (fig. 5). 
No. 2801.—On June 9, a middle-aged 
healthy mare, weighing 850 lbs., was 
given intravenously 450 c. c. of whole 
blood from No. 2597. On June 6 and 
July 3, respectively, 400 c. c. of blood 
were injected. On August 13 and Sep¬ 
tember 12, respectively, 500 c. c. of 
blood were injected. The first symp¬ 
toms were noticed on September 13, a 
brownish-red nasal discharge, weakness, 
and general indisposition. The animal 
radually became worse and died on 
eptember 29. The hemoglobin and 
red cells decreased slightly during the 
illness of the animal (fig. 6). 
No. 2586.—On November 12, a 
healthy mare 10 years of age, weighing 
939 lbs., was given intravenously 60 
c. c. of mixed defibrinated blood from 
Nos. 2316, 2317, and 2318, three sus¬ 
pected field cases. On November 25, 
she was given 30 c. c. more of defi¬ 
brinated blood from No. 2317 and No. 
2318. The first symptoms were noticed 
two weeks after this last injection, and 
the animal died on December 12 (fig. 7). 
