686 
Journal of Agricultural Research 
Vol. XXX, No. 7 
No. 2796.—On June 4, a healthy 
gelding 10 years of age, weighing 1,085 
lbs., was given intravenously 800 c. c. 
6 
a 
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6 
£ 
of blood serum from No. 2913, a typical 
field case. On June 19 this animal was 
given 500 c. c. more of serum from the 
same field case. The first symptoms of 
disease were noticed on June 24. The 
animal slowly grew worse until about 
August 15 when he became very ill and 
remained so until September 1. He 
gradually improved and remained well 
from about September 12 until Feb¬ 
ruary 19, when he was killed. During 
the animal's illness the red cells de¬ 
creased, the hemoglobin was below 
normal and the lymphocytes were 
above normal. Later they came back 
to normal and remained so until the 
animal was killed. Figure 8 shows the 
temperature record of this horse. 
From June 4 until July 27, and from 
September 30 until he was destroyed, 
the temperature was normal. 
No. 2676.—On February 19, a 
healthy gelding 12 years of age was 
given intravenously 750 c. c. of whole 
blood from No. 2597, an experimental 
case produced from a typical field case 
by Berkefeld filtrate. The first symp¬ 
toms were noted on February 27. 
Later the mucous membranes became 
pale and tinged with yellow and there 
was some serous nasal discharge. The 
condition of the animal gradually 
became worse and he died on March 23. 
The red cells and hemoglobin showed 
about a 25 per cent reduction during 
the illness of the animal (fig. 9). 
Since the disease was found to be 
transmissible to susceptible animals 
by inoculation of whole and defibri- 
nated blood, it was desired to see if it 
could be transmitted by the injection 
of Berkefeld-filtered material. Ac¬ 
cordingly, blood was drawn from some 
of the field and experimental cases, 
Berkefeld-filtered, and injected into 
susceptible animals. Before use the 
filters were tested with known cultures 
of bacteria to check their efficiency. 
No. 2008.—On November 2 a healthy 
mare 12 years of age, weighing 925 
lbs., was given intravenously 10 c. c. 
of Berkefeld-filtered blood serum from 
experimental case No. 1948. This ani¬ 
mal remained healthy until May 24, 
when many petechiae were noted in 
the nose, with some hemorrhage. On 
June 3 she was definitely ill. She 
gradually became worse, however, 
developing reddish-yellow membranes, 
serous nasal discharge, edema of the 
legs and abdomen, and loss of weight, 
weighing 748 lbs. She died on July 
14. Very little change was noted on 
blood examination until the late stages 
of the disease, when there was about 
a 20 per cent reduction in the hemo¬ 
globin and red cells (fig. 10). 
No. 3036.—On January 28 a healthy 
mare 14 years of age was given intra- 
