Apr. 1,1925 
Investigations of Infectious Equine Anemia 
687 
venously 100 c. c. of Berkefeld-filtered 
blood serum from No. 2597, an experi¬ 
mental case produced with filtrate. 
The first symptoms were noted on 
February 20. The progress of the 
disease was very rapid, and the animal 
died on February 22 (fig. 11). 
No. 2597.—On November 10 a 
healthy gelding 2 years of age, weigh¬ 
ing 650 lbs., was given 30 c. c. of Berke- 
feld-filtrate from experimental case No. 
2563. On November 20 he was given 
intravenously 30 c. c. of filtrate of 
blood and splenic emulsion and 40 
c. c. more on the following day. On 
January 16 he was quite ill and re¬ 
mained so for some time, gradually 
recovering and being apparently well 
by February 15, when he weighed 732 
lbs. About March 18 he had a re¬ 
lapse and was very ill for a week. 
His weight fell to 573 lbs. At this time 
he was so ill that he was expected to 
die, but he gradually recovered and 
no time was there any evidence of a 
reaction in one of these animals. 
Attempts were made to reproduce 
the disease in animals other than 
horses, but all of these failed. Com¬ 
mon house mice, rabbits, guinea pigs, 
calves, sheep, and pigs were injected 
with blood and kept under careful 
observation for periods varying from 
weeks to several months, all with 
negative results. 
DISCUSSION 
This report can add little to the 
autopsy findings reported by Mack 
(6 ), with the exception of those in 
the long bones. Many writers have 
considered that the findings in the 
bone marrow are characteristic of the 
disease. In a previous paper the 
writer (18) has quite conclusively 
shown that these changes are not 
characteristic and may even be greater 
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was apparently well by the middle of 
April. From this time until he was 
killed on February 26, nearly a year 
later, he showed no departure from 
the normal. During this time, how¬ 
ever, he was an active carrier of the 
virus, many subinoculations being 
made from him, some of which are 
reported here. Figure 12 shows his 
temperature record. During the break 
in his temperature record, and from 
April 11 until his death, the tempera¬ 
ture was normal. During the illness 
of this animal the hemoglobin and red 
cells were decreased about 20 per cent 
and the lymphocytes went as high as 
54.4 per cent. 
As a check on the diagnosis, and in 
order to eliminate acute bacterial 
diseases, blood was taken at nearly 
every autopsy for subcutaneous and 
intraperitoneal inoculations on rabbits 
and guinea pigs. From time to time 
emulsions from the liver, spleen, and 
lymph glands were also injected. At 
in other diseases than in infectious 
equine anemia. 
At all autopsies cultures were made 
on neutral plain agar and neutral 
plain broth. At autopsies and at 
times during the acute illness of the 
various cases cultures were made on 
special media. These media included 
normal rabbit blood agar; 10 per cent 
horse serum, 2 per cent agar and 
physiological saline; 15 per cent horse 
serum and water; 5 per cent horse 
serum and water; 15 per cent horse 
serum and physiological saline; 5 
per cent horse serum and physiological 
saline; 25 per cent horse serum and 
water; 12 per cent horse serum and 
water; 25 per cent horse serum and 
physiological saline; 12 per cent horse 
serum and physiological saline. Some 
of these cultures were oiled in order 
to secure anerobic conditions, a piece 
of rabbit testicle being added in some 
cases and a piece of rabbit kidney in 
others. All cultures were incubated 
