688 
Journal of Agricultural Research 
Vol. XXX, No. 7 
at 37.5° C. for varying periods of 
time, some as long as two months. 
At no time was there any growth that 
could be considered anything but 
contamination from outside sources. 
These results do not agree with those 
of Mivagawa, Taniguchi, Nagao, and 
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Takemoto (11), who were working 
with what is supposed to be the same 
disease in Japan. These investigators 
claim that spirochaetes can be ob¬ 
served in direct smears from the blood 
stained with Giemsa’s stain. Almost 
without exception the blood smears 
made by the writer have been so 
stained, but at no time has anything 
resembling a spirochaete been seen 
in them. This statement is based 
on hundreds of examinations made 
over a period of years. At various 
times fluids from the edematous areas 
also was smeared and stained for 
spirochaetes by the silver and India- 
ink methods, but the results obtained 
were negative. 
One thing that stands out conspicu¬ 
ously in the severest cases of anemia 
is the lack of erythroblasts. These are 
seldom encountered and then only in 
small numbers. Apparently the re¬ 
generative powers of the animals are 
unable to cope with the loss of red 
blood cells. Perhaps this lack of re¬ 
generation or red cells is one of the 
causes of the acuteness of the disease, 
although the chronic cases are the ones 
that show the most pronounced ane¬ 
mia, the acute cases in many instances 
\ 
Fig. 7 —Temperature record of animal No. 2586 
This lack of ability to regenerate red 
cells may be one of the reasons why 
there are no marked changes in the 
bone marrow. One would not expect 
to find extensive marrow changes 
unless there was active blood re¬ 
generation. 
There seems to be a greater loss of 
red cells in the field cases than in the 
experimental ones. This may be ac¬ 
counted for by the overwhelming 
quantities of blood injected into the 
latter and the consequent acuteness of 
the disease produced. The field cases 
as a whole are apparently less acute 
than the experimental ones. 
During the routine examination of 
the stained smears there have been 
observed the so-called intracorpuscular 
bodies, described by Mack (10). What 
relation these have to the disease in 
question is as yet unsettled. In 
these examinations they have been 
found present in many cases. They 
have also been found in normal horses 
