May is, 1925 Possibility of Sex Control by Artificial Insemination 
905 
Table IV presents the correspond¬ 
ing facts for all females which have 
25 or more offspring of known sex. 
Table IV.— Sex of progeny females 
Sex of offspring 
Devia- 
tion— 
l)am 
prob- 
! Males 
Fe¬ 
males 
Un¬ 
known 
able 
error 
14 
17 
16 
4 
0.8 
3.06 
12.2_ 
-1 30 
5 
21.2_ 
_ 1 17 
21 
12 
.96 
26.1_ 
_ 21 
10 
2 
2. 93 
26.2_ 
21 
14 
.22 
26.4_ 
_ 11 
15 
4 
1.16 
14 
56.3_ 
_ 16 
22 
1 
1.44 
65.5_ . 
17 
16 
4 
. 26 
65.6_ 
_ 17 
13 
8 
1.08 
68.5_ 
12 
18 
3 
1. 62 
293A2_ 
_ 13 
12 
1 
.30 
297.1_ 
_ 15 
13 
12 
. 56 
369.2_ 
_ 14 
16 
5 
. 54 
370.2_ 
_ 14 
16 
1 
.54 
If the observed irregularities in the 
sex ratio were due to the presence of 
sex-linked lethal factors in some of the 
breeding stock, the records of indivi¬ 
dual sires and dams should show this 
fact. Tables III and IV do show a 
number of irregularities, but perhaps 
no more than might be expected upon 
the basis of chance deviation. The 
breeding records of c? 316.1, 912.2 and 
926.1 deviate just far enough from 
expectation that it is possible but not 
necessary to regard them as significant. 
All three animals are now dead and it 
is impossible to apply breeding tests 
to settle the question. 
SEX IN RELATION TO DILUTION OF 
SEMINAL FLUID 
The degree of dilution of the re¬ 
covered liquid has an effect upon 
completeness of separation of the 
liquid and the solids contained in it, 
as already mentioned, but that it 
does not seem to have had an effect 
upon the sex ratio of the offspring 
produced is shown by Table V. The 
figures given for degree of dilution are 
in terms of the volume of Ringer’s 
solution, which was added to the liquid 
recovered. Three volumes was the 
amount usually added, but for various 
reasons, usually to secure enough 
volume to be handled easily, other 
dilutions were sometimes made. 
RELATION OF SPEED AND DURATION OF 
CENTRIFUGING TO SEX OF OFFSPRING 
That the speed at which the centri¬ 
fuging was done did not affect the sex 
of the offspring produced is shown by 
Table VI. The true speed of the 
electric centrifuge was undoubtedly 
somewhat greater than the 1,600 r. p. 
m. and the 2,000 r. p. m. measured, 
because the cover was on during the 
actual centrifuging, and therefore the 
air resistance was less than when the 
speed was being measured with a 
revolution counter. 
Table V .—Degree of dilution and sex 
of offspring 
Degree of dilution 
| Sex of offspring 
i A To In I 1 e_ I Un- 
! V 1 male jknown 
i ! 
1 
! Devia¬ 
tion— 
prob¬ 
able 
error 
None_ 
1 to 2_ 
3 _ 
4 to 5_ 
8 to 12_ 
Uncertain_ 
1 32 
! 21 
! 79 
: 16 
13 
1 22 
i 
28 
27 
1 92 
! 12 
4 
11 
1 
5 
8 
1 
4 
« 
0.77 
1. 28 
j 1.47 
1. 12 
3. 23 
| 2.84 
! 
Table VI.— Speed of centrifuging and 
sex of offspring 
Sex of offspring 
Devia¬ 
Speed 
— 
- 
tion— 
prob¬ 
Male 
Fe¬ 
Un¬ 
able 
male 
known 
error 
1,600 r. p. m. (hand 
centrifuge)_ J 
61 
72 
3 
1.41 
1,600 r. p. m. (elec¬ 
tric centrifuge)_: 
97 
85 
3 
1.32 
2,000 r. p. m. (elec¬ 
tric centrifuge)_ 
io ! 
1 
5 1 
1.91 
Odd and unknown 1 
speeds_ 
4 ! 
6 | 
1 
! 
.91 
That the length of time the liquid 
was centrifuged is not a controlling 
factor is shown by Table VII, which 
gives the sexes of the rabbits produced 
from spermatozoa which had been 
centrifuged for varying lengths of time. 
Table VII.— -Time of centrifuging and 
sex of offspring 
Sex of offspring 
Devia¬ 
tion— 
Time centrifuges 
1 
I prob¬ 
Male 
Fe¬ 
Un¬ 
able 
male 
known 
error 
0 to 2 minutes_ 
22 
0. 23 
2 to 4 minutes.. 
31 
41 | 
1 
1. 75 
4 to 6 minutes_ 
30 
33 ! 
10 
.56 
6 to 10 minutes_ 
75 
62 j 
6 
1. 65 
10 to 15 minutes_ 
7 
5 
6 
.85 
Unknown 
4 
2. 97 
i 
