Aug. i, 1925 Nitrogen Constituents of Celery Plants 295 
Table IV .—Analyses of celery leaves.—Healthy leaves contrasted with leave i 
affected by Septoria leaf spot (Septoria apii) 
[Data expressed in percentage of oven-dried celery leaves] 
Blighted leaves 
Aver¬ 
age 
Healthy leaves 
Aver¬ 
age . 
Total nitrogen- 
Nitric nitrogen _ __ 
4.378 
.2501 
.0552 
4.374 
.2501 
.0621 
4.376 
.2501 
.0598 
5.037 
.285 
.0207 
5.15 
.310 
.0138 
5.12 
5.102 
.297 
.0161 
Ammonia nitrogen... 
.0621 
.0138 
Nitrite nitrogen 
Positive test 
Aver¬ 
age 
Negative test 
Aver¬ 
age 
Total hydrolyzable nitrogen_ 
Distribution of above: 
Acid amide 1 ... 
* 4 TTnmin ” „ _ 
.5175 
.479 
1.897 
3.14 
1.236 
3. 705 
.5175 
3.726 
.5175 
3.715 
.5175 
.479 
1.904 
3.18 
1.189 
4.485 
.69 
.4623 
2.423 
3.46 
1.642 
4.581 
.69 
4.533 
.69 
.4623 
2.406 
3.46 
1.642 
Total Amino___ 
Protein nitrogen... 
Nonprotein nitrogen_ 
1.911 
3.22 
1.156 
3.20 
1.176 
2.39 
3.45 
1.652 
3.47 
1.632 
1 Ammonia nitrogen not subtracted. 
DISCUSSION 
A comparison of the figures for the two leaf diseases of celery 
shows that there is iti the case of each disease a lower total nitrogen 
content in the diseased than in the healthy leaves. This is the 
striking thing in the analysis. Whereas the difference between healthy 
and diseased leaves is somewhat greater with the Cercospora apii 
material than with Septoria apii, this variance is not believed to 
be significant since the extent of the diseased tissue was greater 
with the Cercospora apii material than with that from Septoria 
apii . The consistently lower total nitrogen content is the important 
consideration. 
There is a lower nitric nitrogen content in diseased leaves. The 
difference is not significant in the case of Septoria apii material, but 
is pronounced in the case of Cercospora apii . 
Ammonia nitrogen, in the case of both the Cercospora apii and the 
Septoria apii tests, was greater iti the diseased material than in the 
corresponding healthy material. While the differences between* 
healthy and diseased material is less in the case of Septoria apii than 
with Cercospora apii , this is not to be taken as a characteristic differ¬ 
ence always to be expected. It is rather to be referred to the per¬ 
centage of diseased material present in the samples. It has com¬ 
monly been noticed that with leaves extensively affected with 
Septoria apii an ammonia odor is very noticeable. 
With Cercospora apii material, nitrite was definitely shown to be 
present by using Jodidi’s technique. It was only faintly evident in 
the healthy, control material. Nitrite could not be so demonstrated 
a with Septoria apii material, although tested repeatedly. Bv a differ¬ 
ent method of extraction, however, abundant evidence of presence 
of nitrites was obtained. 
With Cercospora apii material, the diamino acid content was 
determined, the monoamino nitrogen being obtained by difference. 
The healthy leaflets showed the greater per cent of these compounds. 
With Septoria apii, total aminoacid nitrogen was determined, and 
here also the amount in the healthy leaflets exceeded that of the 
