Aug. 15, 1925 
Studies on Isoelectric Points for Plant Tissue 
391 
Table IV. — Summary of results with mycelium of Gibberella saubinetii . Tissue 
in each case placed in solution indicated and reaction measured until equilibrium 
was reached 
Experi¬ 
ment 
No. 
Weight 
of myce¬ 
lium 
Age of 
myce¬ 
lium 
Kind of buffer solution used 
Quantity 
of buffer 
used 
Initial 
Ph 
Final 
Ph 
Time 
required 
to reach 
equilib¬ 
rium 
1,_1 
Grams 
5. 67 
Days 
15 
0.002 M. Na phosphates_ 
C. c. 
50 
4.03 
6.14 
Minutes 
60 
2__. 
4 40 
15 
0.001 M. Na phosphates_ 
50 
4.37 
6.05 
65 
3_ 
4.49 
16 
0.001 M. KH phthalate+Hs 
25 
3.15 
6.23 
21 
4_ 
1. 51 
16 
P0 4 . 
0.001 M. Na phosphates _ 
25 
5.88 
6.25 
19 
5_ 
1.88 
11 
0.001 M. Na phosphates_ 
25 
6.68 
6.21 
77 
6_ . 
4.25 
14 
0.001 M. Na phosphates. _ 
- 50 
7.88 
6.34' 
126 
7_ 
4.65 
16 
0.001 M. Na phosphates_ 
50 
7.42 
6.36 
103 
Fig. 1.—Curves showing the effect of the mycelium of Gibberella saubinetii upon the reaction of dilute buffer 
mixtures P H of the buffer mixture is plotted against the time. The numbers on the curves refer to the 
experiments summarized in Table IV 
The effect of the mycelium of G . saubinetii upon the dilute buffer 
mixtures was similar to that produced by an ampholyte with an 
isoelectric point near P H 6.2. Solutions of initial P H 3.15, 4.03, 4.37, 
