Augi 15,1925 
Studies on Isoelectric Points for Plant Tissue 
393 
MYCELIAL MATS OF FUSARIUM LYCOPERSICI 
The results obtained with the mycelial mats of Fusarium lycoversici 
are summarized in Table V. In Figure 2, curves of the results are 
given with the P H of the solutions on the ordinate and time on the 
abscissa. 
The mycelium of F . lycoversici affects the buffer mixtures used 
like an ampholyte with an isoelectric point at P H 5.5. Solutions of 
initial reaction P H 3.94, 3.95, 4.03, 4.55, and 5.43 became more 
alkaline because of the presence of this fungus, while solutions of 
initial P H 7.20 and 6.66 became more acid. The results of experi¬ 
ment 9 can be overlooked in drawing conclusions, because the 16- 
to the experiments summarized in Table V 
day old mycelium used in this experiment was badly autolyzed. 
Disregarding this experiment, the lowest P H reached from the alkaline 
side was P H 5.52, and the highest from the acid side was P H 5.50. 
Again the much greater rapidity of action of the mycelium in 
changing the reaction of phthalate buffer mixtures, as compared to 
the phosphate solutions occurred. In experiment 4 it required 16 
minutes tor 1.50 gms. of the mycelium to change an 0.01 M. phthalate 
solution of P„ 3.95 to a P„ of 5.41; while, in experiment 2, 2.14 
gms. of mycelium required 70 minutes to make a smaller change in 
the reaction of a 0.002 M. phosphate buffer mixture. This differ¬ 
ence in speed of action is brought out clearly by the much steeper 
slope of the curve for experiment 4 as contrasted with that for 
experiment 2, as shown in Figure 2. 
