394 
Journal of Agricultural Research 
Vol. XXXI, No. 4 
The changes in reaction in experiment 6 did not follow the regular 
order of the balance. Instead of proceeding uniformly toward an 
equilibrium point, the mycelium changed the reaction to above 
P H 5.50, then to a point below that value, followed by a second 
oscillation toward greater alkalinity before the reaction settled to 
an equilibrium point at P H 5.48. This is shown by the curve for 
experiment 6 in Figure 2. The initial reaction of the solution in 
experiment 6 was more nearly the isoelectric point than any other 
used. This fact may be connected with the peculiar action of the 
mycelium in this experiment. 
r/AfB //V Af//V(/T£S 
Fig. 3.—Curves showing the effect of the mycelium of Fusarium oxysporum upon the reaction of dilute 
buffer mixtures. The Ph of the buffer mixture is plotted against time. The numbers on the curves refer 
to the experiments summarized in Table VI 
The isoelectric point found for the mycelium of Fusarium 
lycopersici in these experiments agrees very well with that found 
earlier in studies on the influence of hydrogen-ion concentration on 
dye absorption (6), and on the growth of the same strain of this 
organism {8). 
MYCELIAL MATS OF FUSARIUM OXYSPORUM 
The results of experiments with Fusarium oxysporum are sum¬ 
marized in Table VI and Figure 3. 
The mycelium of F. oxysporum acts much like an kmpholyte with 
an isoelectric point near P H 4.9. Solutions of initial P H 3,80, 4.02, 
4.04, and 4.73 became more alkaline because of the presence of the 
mycelium; while solutions of initial P H 7.36, 7.10, 6.94, 5.70, and 
5.06 because more acid. The highest final P ? reached from the 
acid side was 4.94, and the lowest from the alkaline side was 4.71. 
