668 
Journal of Agricultural Research 
Vol. XXXI, No. 7 
Table III shows the temperature reactions and variations in milk 
yield that followed the initial treatment. They are more or less 
a ical of the temperature and miJk yield variations that occurred 
owing the subsequent bacterin injections. 
Table III. — Temperatures and milk yield of cows for four days following first 
abortion-bacterin treatment on May 24, 1922 
Cow 
No. 
Date 
8 a. m. 
12 m. 
4.30 
p. m. 
Milk 
yield 
Cow 
No. 
Date 
8 a. m. 
12 m. 
4.30 
p. m. 
Milk 
yield 
o jp 
° F. 
° F. 
| Pounds 
o F 
o p 
° F. 
Pounds 
51 
May 24.. 
101.0 
101.1 
46.7 
435 
May 24.. 
101.0 
29.1 
May 25.. 
103.7 
103.2 
103.0 
43.3 
May 25._ 
101.8 
101.6 
101.4 
25.9 
May 26.. 
103.2 
101.6 
101.8 
45.3 
May 26.. 
101.6 
101.8 
101.7 
29.8 
May 27.. 
101.8 
101.7 
102.0 
44.6 
May 27.. 
101.5 
101.4 
101.8 
29.5 
May 28.. 
101.9 
102.0 
102.0 
47.3 
May 28.. 
101.2 
101.2 
101.0 
28.2 
247 
May 24.. 
100.6 
52.5 
439 
May 24. _ 
100.8 
100.8 
16.8 
May 25.. 
103.4 
103.0 
101.4 
49.7 
May 25. _ 
101.5 
101.8 
101.5 
17.7 
May 26.. 
101.0 
101.4 
101.5 
50.9 
May 26.. 
101.6 
101.5 
101.6 
16.3 
May 27.. 
101.2 
101.2 
100.6 
52.2 
May 27.. 
101.7 
101.7 
101.8 
18.6 
May 28.. 
101.0 
101.2 
101.0 
51.0 
May 28.. 
101.6 
101.7 
101.8 
17.5 
442 
May 24 
101.2 
23.9 
409 
May 24.. 
101.0 
$2.9 
May 25.. 
101.8 
101.6 
101.4 
22.6 
May 25.. 
100.8 
100.9 
101.0 
3L0 
May 26.. 
101.0 
101.3 
101.2 
23.2 
May 26.. 
100.6 
100.9 
'101.3 
33.1 
May 27.. 
101.2 
101.2 
101.4 
23.2 
May 27. _ 
101.2 
101.2 
101.0 
31.6 
May 28.. 
101.5 
101.4 
101.3 
22.8 
May 28.. 
101.3 
101.4 
101.0 
31.1 
132 
May 24 
101.2 
22.0 
468 
May 24. _ 
100.4 
100.3 
21.4 
May 25.. 
102.0 
101.8 
101.5 
19.3 
May 25.. 
100.8 
101.3 
101.4 
22.2 
May 26.. 
101.2 
101.2 
101.5 
19.4 
May 26_. 
101.9 
101.7 
101.8 
20.3 
May 27.. 
101.1 
101.2 
101.4 
21.5 
May 27.. 
101.8 
102.0 
102.0 
20.9 
May 28.. 
101.5 
101.4 
101.0 
20.8 
May 28.. 
101.8 
102.0 
101.9 
22.2 
Composite samples of milk were obtained from animals of the 
treated and control groups on six different dates following the admin¬ 
istration of the bacterin, with the object of determining whether the 
bacterin injections had exerted curative action. Blood samples 
were also usually taken on the same dates. The blood and milk 
samples collected on the various dates were handled in a manner 
similar to that described for the original collection. The periods 
that intervened between the completion of the bacterin treatments 
and the collection of the different samples were approximately 2, 4, 
6, 11, 15, and 20 months. 
Tables IV to XII, inclusive, show the manner in which the blood 
and milk samples reacted to the agglutination test, results of the milk 
inoculations of guinea pigs, the serological and cultural results ob¬ 
tained from the inoculated guinea pigs, and other data. 
DISCUSSION OF RESULTS 
In the writers’ studies of the results of numerous gumea-pig inocu¬ 
lations it was found that the nature of the agglutination results of 
the blood serum, five to eight weeks after inoculation, was in most 
cases a reliable index to the presence or absence of Bacterium abortus 
infection, and that to depend wholly upon macroscopic lesions as 
indicators of such infections may in many cases prove misleading. 
This was clearly evidenced by the fact that it was frequently possible 
to isolate from 150 to 200 colonies of the abortion organism from 
spleens of inoculated guinea pigs which were normal in their gross 
arance. In only a few instances did reacting guinea pigs give 
ive cultural results. 
