Oct. 15,1925 Translocation of Food Materials of Wheat Seedlings 731 
The green weight, dry weight, and ash determinations, as shown in 
Table V and plotted in Figure 1, were made on plants grown as just 
described. The plants were counted, dried of excess moisture, placed 
in weighing bottles, then weighed to determine the green weight, and 
then dried rapidly in a vacuum oven at 80° C. to complete desiccation. 
The dried plants were then transferred to porcelain crucibles and 
reduced to constant weight for ash in an electric muffle furnace at 
dark-red heat. The ash determinations are expressed not only as 
grams of ash but also per cent ash of dry weight and per cent of 
weight of the original seed. 
Table V. —Green weighty dry weighty and weight of ash for 100 parts , expressed in 
grams , and per cent of ash in dry material, per cent of ash based on ash of original 
seedy and per cent of dry weight of original seed 
Age and plant 
Part of 
green 
weight 
Dry 
weight 
Weight 
of ash 
per 100 
parts 
Per cent 
ash of 
dry 
weight 
Per cent 
ash of 
original 
seed 
Per cent 
dry 
weight of 
original 
seed 
Original seed (air dry)___ __ ___ 
2.8990 
2.6228 
0.0534 
2.03 
Plumule: 
3-day_ 
.4760 
.0896 
.0059 
6.58 
11.0 
3.42 
6 -day.... 
3.8260 
.3210 
.0188 
5.84 
35.2 
12.24 
9-day.... 
6.4740 
.6401 
.0534 
7.22 
100.0 
24.40 
12 -day...... 
8.7840 
.8428 
.0910 
10.75 
170.2 
32. 13 
15-day_ 
14.2085 
1.3961 
.1869 
11.94 
350.0 
53.22 
25-day____ 
32.2880 
3.2612 
.3572 
10.95 
668.9 
124.30 
Roots: 
3-day__.... 
.851 
.0819 
.0063 
7.73 
11.8 
3.12 
6 -day__ 
3.860 
.2460 
.0229 
9.31 
42.89 
9.37 
9-day....... 
6.578 
.4521 
.0465 
10.27 
87.08 
17.23 
12 -day.... 
9.520 
.4889 
.0628 
12.85 
117. 61 
18.64 
15-day__ 
12.3648 
.5990 
.0659 
10.99 
123.41 
22.83 
25-day..... 
16.787 
1.11850 
.0976 
8.25 
182. 77 
45.18 
Seed: 
3-day... 
4.284 
2.3632 
.0471 
1.95 
88.20 
91. 62 
6 -day__ 
2.440 
1.8072 
.0316 
1.74 
59.18 
68.90 
9-day..... 
3.066 
.8694 
.0141 
1.63 
26.40 
33.15 
12 -day_ 
1.995 
.3384 
.0095 
2.79 
17.79 
12.52 
15-day_ 
2.014 
.2622 
.0062 
2.36 
11 . 61 
9.99 
25-day.... 
1.308 
.2432 
.0088 
3.60 
16.48 
9. 26 
Plant: 
3-day..... 
5.611 
2.5745 
.0593 
2.30 
111. 05 
98.16 
6 -day ...... 
10.126 
2.3742 
.0733 
3.09 
137. 27 
90.52 
9-day....... 
16.118 
1.9616 
.1140 
5.81 
213.48 
74.79 
12 -day...-.. 
20.299 
1.6701 
.1633 
9.77 
305.60 
63.71 
15-day.... 
28.5873 
2.2513 
.2590 
11.50 
485.02 
85.83 
25-day...... 
50.383 
4.6894 
.4636 
9.88 
868.15 
178.80 
The plants used for the chemical analysis were separated into seeds, 
plumules, and roots. The material was dried in a vacuum oven at 
80° C., which has been found by Link and Tottingham (30) to be a 
very successful way to treat carbohydrate material for the analysis 
of sugars. In cases where the sugar content was high, as for example 
in seeds from the 6 and 9-day-old seedlings, it was found necessary, 
even under these conditions, to use Petri dishes and have the seeds 
well separated instead of using the weighing bottles which were used 
in the other cases, in order to have the drying go on fast enough to 
prevent discoloration and acccompanying chemical changes. It was 
also necessary to avoid putting large quantities of material in the 
oven at bne time (an amount which would dry enough in six hours 
to crumble nicely seemed to give the best results). In cases where it 
was dried more slowly it usually lost the bright, desirable color. 
This method is preferable to the alcohol method where small quantities 
