1154 
Journal of Agricultural Research 
Vol. XXXI, No. 12 
lings of the clean-looking rows were weighed, then dried to constant 
weight at 105° C. and weighed again. The results are recorded in 
Table II. 
Table II .—Change of weight in the various seedlings during germination 
No. 
Conditions of germination 
Weight of unger¬ 
minated seed (10 
kernels) 
Weight 
of oven- 
dried 
seedlings 
Unger¬ 
minated 
oven- 
dried 
seed 
Dura¬ 
tion 
Substratum 
Temper¬ 
ature 
Treatment 
Air-dry 
Oven- 
dried 
Days 
° C. 
Grams 
Grams 
Grams 
Per cent 
1 
2 
Paper__ 
25 
None_ 
3.8200 
3.6080 
3.4914 
96. 77 
2 
2 
--do_ 
25 
-do_ . . 
3. 7408 
3.5332 
3.4088 
96.48 
Average.. __ 
96.63 
3 
4 
‘Paper_ 
25 
None_ _ 
3.7200 
3. 5135 
3.2460 
92.39 
4 
4- 
_‘do_ 
25 
_do_ 
3.67$4 
3.4742 
3.2034 
92.21 
Average_ 
92.30 
5 
5 
Paper... 
25 
None_ __ 
3.9100 
3.6930 
' 3.2746 
88.67 
6 
5 
_‘.do___ 
25 
_do_ 
3.9637 
3. 7437 
3.3631 
89.83 
Average_ j_ 
89.25 
7 
8 
Paper_ 
25 
None_ 
3.6916 
3.4867 
2.8093 
80.57 
8 
8 
_do_ 
25 
_do___ 
3.8727 
3.6578 
3.0856 
84.36 
Average_ 
82.47 
9 
2 
Paper__ 
25 
Uspulun.. _ _ _ 
3.9900 
3. 7686 
3.6281 
96. 27 
10 
4 
_do_ 
25 
_‘.do_ 
3. 7891 
3.5788 
3.3104 
92.50 
11 
5 
_do_ 
25 
_do_ 
3.4386 
3. 2478 
2.9208 
89.93 
12 
8 
25 
__do_ 
3. 5213 
3.3259 
2.8015 
84.23 
A glance at Table II shows that after 2, 4, 5, and 8 days of germina¬ 
tion the weight of the seed was reduced, respectively, to 96.63, 
92.30, 89.25, and 82.47 per cent. Equally, in the case of the uspulun- 
treated seed, it decreased to 96.27, 92.50, 89.93, and 84.23 per cent, 
after 2, 4, 5, and 8 days respectively. By means of this table the 
recalculation of the total nitrogen to the oven-dried seed (column 7 of 
Table I) is very simple. Thus, for example, No. 6 (Table I, column 
6) which, after 2 days' germination was found to contain 1.77 per 
cent of nitrogen, calculated on the basis of the oven-dried seedlings, 
has 1.77X0.9663, or 1.71 per cent of nitrogen calculated on the 
oven-dried seed. The same considerations apply to the other 
tables. 
AQUEOUS EXTRACTS OF THE SEEDLINGS 
Although the results presented in Table I clearly establish the 
fact that the compounds constituting the nonprotein nitrogen are 
increasing with the progress of the germination, yet it seemed desir¬ 
able to corroborate this finding by direct extraction with water, 
which, moreover, was necessary for the succeeding experiments. 
Accordingly, aqueous extracts were prepared as follows. Ten-gram 
portions of flour of the various seedlings were treated with 200 c. c. 
of boiling hot ammonia-free water and kept on a water bath for 30 
minutes, after which the whole was centrifuged or filtered. The 
solid residues were treated once more in like manner. Both extracts 
were combined and made up to 500 c. c., of which two portions of 
