CULTURAL CHARACTERISTICS 
Agar Slant.—The growth on agar slant is abundant, beaded, raised, dull, 
smooth, opaque, non-chromogenic, and gives off a decided pungent odor after 
24 hours incubation. The growth is semi-viscid and the medium remains un¬ 
changed. Sufficient growth for observation is usually obtained after 16 to 18 
hours. 
Agar Colonies.—Colonies on agar plates make rapid growth, are puncti- 
form in form, smooth, raised, entire and finely granular in consistency. 
Gelatin.—In gelatin stabs the growth is uniform along the site of stab, 
beaded, non-liquifying, with the medium remaining unchanged. 
FIG. 4.—PHOTOMICROGRAPH OF EBERTHELLA SANGUINARIA 
Potato.—On potato there is usually a moderate growth, yellowish- 
brown is color in 24 hours. 
Nutrient Broth.—There is no surface growth; moderate clouding in 24 
hours emitting a distinct penetrating odor, and producing a scanty flaky 
sediment. 
Temperature Relations.—The optimum temperature for growth of 
E. sanguinaria is 37% degrees C. The maximum temperature at which suit¬ 
able growth is secured is 44 degrees C., while a fairly good growth is produced 
at 18 degrees C. in 72 hours. The thermal death point of the organism is 62% 
degrees C. for ten minutes, and it resists dry heat on glass rods for 5 minutes 
at 75 degrees C., but is killed at the same temperature when heated for 10 
minutes. 
Relation to Reaction of the Medium.—On agar, the optimum hydrogen 
ion concentration is about pH 6.4. The organism has a high tolerance to 
organic acids, suitable growth being obtained on agar acidified with malic 
acid, with oxalic acid, and with formic acid between the ranges of pH 4.8 
and 7.0. 
