Fowl Typhoid and Fowl Cholera 
19 
As indicated, no gas was formed in any of these fermentation tests. This 
result agrees with all available scientific literature on this subject as to the 
lack of ability of E. sanguinaria to form gas in any known carbohydrate or 
higher alcohol. Rettger and Koser (26) tried experimentally to bring about 
gas production with sanguinaria by altering the compositions of medias, but 
were unsuccessful. 
FIG. 5.—CROSS-AGGLUTINATION. ANTI-PULLORA SERUM V. TYPHOID ANTI SEN 
The tendency of sanguinaria to produce a slight alkalinity in Lactose is in 
agreement with the work of Taylor, Muslow, Goldberg (8) and Rettger and 
Koser. 
With the exception of the reaction in saccharose the results obtained in the 
carbohydrate reactions agreed with those of Rettger and Koser, Hadley, Mus¬ 
low, Moore, and Goldberg. After 5 days of incubation we were unable to 
demonstrate neutrality in saccharose with tests on 54 strains. In some in¬ 
stances, however, the reaction produced was nearer neutrality than the aver¬ 
age would indicate. 
FIG. 6.—CROSS-AGGLUTINATION. ANTI-AVIAN TYPHOID SERUM V. PULLORA 
ANTIGEN 
Methyl Red Test.—E sanguinaria is methyl red positive in Maltose 
broth after 48 hours incubation. 
Pathogenicity.—The domestic fowl, turkey, guinea, pigeon, duck, and 
geese are susceptible to E. sanguinaria, these birds being susceptible to labora¬ 
tory infection. Sparrows are susceptible to subcutaneous inoculation. White 
rats and mice are mildly susceptible to subcutaneous inoculation, but sue- 
