20 
North Carolina Experiment Station 
cumb to intraperitoneal injection within 5 days. Guinea pigs succumb to 
intraperitoneal inoculation, but resist subcutaneous injection. Rabbits suc¬ 
cumb to small doses of the organism. 
Muslow reports rabbits, guinea pigs, cats, rats, mice, sparrows, pigeons and 
chickens non-susceptible to laboratory cultures of B. pullorum and B. sangui- 
narium when administered by the mouth. This does not agree with the find¬ 
ings of this laboratory in regard to experimental infection of chickens and 
pigeons. 
FIG. 7.—CROSS-AGGLUTINATION. ANTI-AVIAN TYPHOID SERUM V. AVIAN 
TYPHOID ANTIGEN 
Agglutination.—Agglutination tests with B. typhosus, Paratyphoid A, 
Paratyphoid B, S. pullora and E. sanguinaria show an antigenic relationship 
between these organisms. This phenomena was first observed by Smith and 
Ten Broeck. The results of these agglutination tests are shown in Table II, 
and are graphically presented in Figures 5, 6, 7. The anti-pullorum serum 
used in these tests was secured through the courtesy of Dr. Rettger, and the 
anti-avian typhoid serum was developed in this laboratory. The results ob¬ 
tained in these tests approximate the findings of Smith and Ten Broeck, Rett¬ 
ger and Koser, Hadley, Muslow, and Krumweide and Kohn. 
Table II 
Agglutination With Immune Serum to E. Sanguinaria Titer 1:5000 
Control 
1:10 
1:25 
1:50 
1:100 
1:500 
1:1000 
1:5000 
1:10000 
B. tvphosus. _ __ 
— 
++++ 
"b+~b+ 
++++ 
++++ 
++++ 
++++ 
B. paratyphosus A_ 
— 
H—1—1—b 
b—1—!—b 
++++ 
++++ 
++++ 
++ 
— 
— 
B. paratyphosus B.. 
— 
H—1—1—b 
++++ 
++++ 
+-H-+ 
+++ 
+ 
— 
— 
S. pullora_ 
- 
H—1—1—b 
P+++ 
H—b~l—b 
| | | | 
-1—1—1—b 
1 ! 1 
Agglutination With Immune Serum to S. Pullora Titer 1:1000 
B. typhosus_ _ 
_ 
++++ 
++++ 
++++ 
++++ 
+++4 
++++ 
B. paratyphosus A_ 
— 
++++ 
++++ 
H—b 1—b 
H—1—b + 
++++ 
++ 
— 
— 
B. paratyphosus B_ 
— 
++++ 
++++ 
++++ 
++++ 
+ + 
— 
— 
— 
E. sanguinaria. 
H — 1 — 1 — b 
H — 1 — 1 — b 
b — b”b+ 
++++ 
++ + + 
++++ 
— 
— 
