48 North Carolina Experiment Station 
Clinical Data 
j Date 
Respiration Temperature 
Remarks 
Feb. 27 
35 
107.8 
Pre-inoculation 
28 
33 
107.0 
Pre-inoculation 
Mar. 1 
33 
107.5 
Day of inoculation 
2 
32 
108.2 
Bird normal 
3 
30 
110.2 
Whitish dejecta; depression 
4 
28 
109.8 
Watery discharge 
5 
28 
109.7 
Sulphurus discharge 
6 
30 
108.0 
Extreme depression 
7 
30 
109.0 
Extreme depression 
8 
29 
108.6 
Extreme depression 
9 
30 
108.2 
Extreme depression 
10 
28 
110.0 
Partial condition of coma 
11 
27 
107.5 
Partial condition of coma 
12 
20 
107.0 
Partial condition of coma 
The bird 
died during the 
morning of 
the 12th. E. sanguinaria recovered 
from the liver, spleen, kidneys and bone marrow. Heart and lung cultures 
negative. 
The disease was successfully produced by subcutaneous inoculation as 
shown in the following clinical study: 
Subject.—Rhode Island Red hen. 
Source.—Central plant. 
Condition on Infection.—Bird normal. 
Method 
of Infection.—2 
cc. of a 24 
l-hour bacillary saline emulsion of 
American sanguinarium 67 given subcutaneously March 5, 1923, at 10:30 a. m. 
Date 
Clinical 
Respiration Temperature 
Data 
Remarks 
Mar. 3 
38 
106.4 
Pre-inoculation 
4 
36 
107.0 
Pre-inoculation 
5 
42 
108.0 
Day of inoculation 
6 
35 
108.0 
Bird normal 
7 
42 
108.8 
Slightly depressed 
8 
36 
108.0 
Slightly depressed 
9 
36 
108.8 
Slightly depressed 
10 
42 
108.3 
Bird dejected 
11 
36 
109.5 
Watery discharge 
12 
31 
109.3 
Watery discharge 
13 
30 
108.8 
Watery discharge 
14 
34 
108.1 
Watery discharge 
15 
30 
107.8 
Sulphur colored discharge 
16 
36 
108.6 
Bird depressed 
17 
30 
107.8 
Bird depressed 
18 
30 
107.8 
Bird depressed 
19 
30 
107.8 
Bird depressed 
20 
30 
108.2 
Bird depressed 
The bird died at noon on the 20th. E. sanguinaria isolated from the heart, 
liver and spleen. 
The most successful method of infecting birds with avian typhoid in these 
experiments was by infection per orum. In this method of infection the 
period of incubation seemed relatively constant, and this method was used in 
blood study work in order to secure a maximum chance of take, and eliminate 
