110 
N. C. AGRICULTURAL EXPERIMENT STATION. 
TABLE II—SHOWING RELATION OF TEMPERATURE AND GROWTH. 
Temperature 
—Compartment Number 
t Growth—Compartment Number 
1 
2 
3 
4 
5 
6 
1 
2 
3 
4 
5 
6 
Date 
Max. Min. 
Max. Min. 
3-14-08... 
13 16 
21 
24 
26 
29 
36 
39 
0 
4 
5 
3 
4 
0 
3-16-08... 
13 21 
23 
25 
27 
29 
36 
39 
10-15 
16-21 
20 
17 
16 
0 
1 
1 
CO 
o 
1 
r- 
T-H 
1 
co 
13 23 
21 
24 
26 
29 
36 
39 
0-10 
13-15 
13 
10-15 
7 
0 
3-18-08... 
13 17 
21 
24 
26 
29 
36 
39 
5-10 
13-16 
14-17 
0- 5 
8 
0 
3-19-08... 
14 17 
• 
21 
24 
26 
29 
36 
39 
7-27 
13-21 
5- 9 
2 
5 
0 
3-20-08... 
13 16 
21 
24 
26 
• 
29 
36 
39 
8-25 
F* 
F 
8 
5 
0 
3-21-08... 
14 17 
21 
24 
26 
29 
36 
39 
5-10 
F 
F 
10 
5 
0 
3-23-08... 
13 18 
21 
24 
26 
29 
36 
39 
10 
F 
F 
10 
10 
0 
3-24-08... 
13 16 
21 
24 
26 
29 
36 
39 
10-25 
F 
F 
F 
0 
0 
3-25-08... 
12 18 
21 
24 
26 
29 
37 
39 
F 
St 
S 
S 
0 
0 
3-26-08... 
13 18 
21 
24 
26' 
29 
36 
39 
S 
S 
S 
s 
0 
0 
Average number of sclerotia per culture_ __ 
1 
2 
2 
1.8 
0 
0 
* F indicates date upon which mycelium filled plate, 
t S indicates that sclerotia were forming. 
t Growth for each day is recorded in millimeters. In compartments 1 and 6 maximum and 
minimum thermometers were used. 
Of the four compartments of intermediate temperature the two 
cooler (21 and 24 degrees) were more favorable than the two warmer 
(26 and 29 degrees). In the two former complete occupancy of the plate 
by the mycelium occurred in six days while the others required four 
days more. 
From these tests we may conclude that this fungus can not con¬ 
tinue to grow long at a temperature of 29 degrees or higher; that at 
a temperature as low as 13-18 degrees or as high as 26 degrees growth 
proceeds normally but'not so rapidly as at the optimum temperature; 
which lies between 21 and 24 degrees. 
Experiment 13. To determine longevity of mycelium in agar in 
petri dishes. A ten-per-cent, lettuce agar was employed, acidity -[-.55 
Fuller’s scale. Tests were made in the laboratory, the results of which 
appear in table III. , 
From this table it is seen that the mycelium remained alive as long 
as forty-eight days, but in no case did it live beyond the fifty-fifth 
day. The length of life after the agar was dry in the petri dish 
varied from five to ten days. 
Experiment 12. To determine the effects of various nutrients 
upon growth. Sclerotinia was grown in petri dishes upon plain agar 
with various nutrients added. 10 c.c. of medium was used in each 
dish. The petri dishes were inoculated 12-11-1906. 
