A SERIOUS LETTUCE DISEASE. 
Ill 
TABLE III.—SHOWING LONGEVITY OF MYCELIUM IN AGAR IN PETRI DISHES. 
Plate 
No. 
Date of 
Inoc. 
Date of 
Filling 
Plate 
Date of 
Drying 
tested 
alive 
tested 
alive 
tested 
alive 
tested; alive 
tested 
alive tested 
alive 
1 
5-15-09 
5-21 
6-20 
6-1 
Yes 
6-14 
Yes 
6-23 
Yes 
6-30' Yes 
7- 7 
No L__ 
2 
5-15-09 
5-21 
6-20 
6-1 
( i 
6- 8 
4 i 
6-14 
“ 
6-23 “ 
6-30 
Yes I 7- 7 
No 
3 
5-15-09 
5-23 
6-22 
6-1 
< i 
6-8 
i i 
6-14 
4 4 
6-23 “ 
6-30 
“ 7- 7 
No 
1 
6-14-09 
6-18 
7-18 
6-18 
7- 7 
4 4 
7-14 
7-23 “ 
8- 3 
No _ 
2 
6-14-09 
6-18 
7-18 
6-18 
7- 7 
« t 
7-14 
4 i 
7-23 “ 
8- 3 
4 4 
3 
6-14-09 
6-18 
7-18 
6-18 
7- 7 
i t 
7-14 
4 4 
7-23 “ 
8- 3 
“ _ 
4 
6-14-09 
6-18 
7-18 
6-18 
7- 7 
4 4 
7-14 
4 4 
7-23 “ 
8- 3 
4 4 
1 - - 
1 
9-23-09 
9-27 
10-28 
9-30 
10-14 
4 4 
10-28 
4 4 
11- 7 “ 
11-10 
4 4 
2 
9-23-09 
9-27 
10-28 
9-30 
10-14 
4 4 
10-28 
4 4 
11- 3 “ 
11-10 
4 4 
3 
9-23-09 
9-27 
10-28 
9-30 
10-14 
4 4 
10-28 
4 4 
11- 3 “ 
11-10 
4 4 
As might have been expected growth was very slow in plain agar, 
while 5 per cent, glucose, and 1 per cent, starch proved most favor¬ 
able. Lettuce agar was much superior to plain agar but far less 
nutritious than starch or glucose agars. 
The deficiency of the peptone and lactose agars is striking, seeming 
to emphasize the lack of need of rich nitrogenous foods for this fungus 
and to show the superior value of glucose over lactose as a source of 
carbon. There was no reddening of the litmus lactose agar by the 
mycelium. 
Sclerotia w^ere formed on all of these media except the peptone and 
the litmus lactose agar. 
TABLE IV— SHOWING EFFECTS OF VARIOUS NUTRIENTS UPON GROWTH. 
Growth Is Recorded in Millimeters. 
Medium 
Days 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
1 
Plain agar. _. . __ _ _ __ _ 
5 
9 
14 
20 
23 
25 
30 
30 
39 
2 
Plain agar. . __ . _ _ 
2 
2 
12 
1 6 
25 
32 
35 
36 
43 
3 
Plain agar+1 per cent glucose_ .. . __ 
3 
11 
25 
28 
34 
38 
42 
4 
Plain agar+1 per cent glucose . . _ .. _ 
1 
4 
10 
10 
15 
24 
26 
5 
Plain agar+5 per cent glucose_ . .. ___ 
2 
8 
40 
65 
6 
Plain agar+5 per cent glucose_ _ _________ 
3 
25 
75 
Fills 
Plate 
7 
Plain agar +1 per cent peptone.. . ... 
2 
5 
10 
10 
12 
12 
15 
9 
Plain agar+Litmus lactose . . ___ . 
2 
10 
20 
24 
26 
28 
10 
1 per cent starch agar __ _ ______ 
3 
13 
30 
40 
45 
50 
60 
11 
1 per cent starch agar_ ._ _ 
ft 
8 
25 
42 
50 
58 
12 
Lettuce agar_ . . _ _ 
4 
13 
20 
40 
47 
53 
70 
13 
Lettuce agar . _.. ___ _ 
4 
8 
14 
22 
32 
42 
48 
