122 
N. C. AGRICULTURAL EXPERIMENT STATION. 
The result of these tests are shown in the following table: 
TABLE X.—SHOWING EFFECT OF VARIOUS SUBSTANCES UPON SPORE 
GERMINATION. 
>—* 
o 
o 
O 
O* 
O 
to 
o 
td 
td 
o 
o 
O 
O 
O 0- 
• CD 
° S- 
• CD 
CD 
CD 
(S3 S° 
H- P 
t- 
• e 
X 
• P 
X 
m 
c_ 
w 
p_ 
iS 
V 
t 
>r~ 
<r+- 
P 4 
P 4 
c+- 
P 
<rh 
p 
C-t~ 
c 
CD 
CD 
CD 
CD 
■ 
o_ 
o_ 
*4 
p 
c4- 
c 
r? 
O 
o 
*-i 
P 
c4 
H 
P 
c4 
0 
at 1 
j- 
OO 
CD 
CD 
° p 
o 1 
to 
o 
o 
0 
O 
o 
*0 
CD 
>-< 
U1 
*cf 
er 
p 
EC 
O 
O 
3 
o 
o 
o 
o 
o 
c 
t 
t 
t 
t 
CD 
CD 
M 
CD 
oq 
c 
P 
xs 
n 
p 4 
p- 
EO 
p 
-“4 
c4- 
CD 
CD 
CD 
O 
CD 
fT 
c+- 
P 
c4 
o 
P 
O 
P 
OO 
to 
o 
p 
o 
p 
o 
o 
O 
o 
■o 
ts 
(T> 
i-l 
CO 
c 
'5' 
P 4 
p 
erf- 
CD 
CD 
O 
O 
3 
o 
o 
c-»- 
P 
o 
cd 
w 
o 
r+- 
€© 
to 
p 
p 
c+ 
CD 
OO 
Oi 
C 
*-< 
Q- 
H* 
0 
P 
H 
<< 
GO 
e-4 
CD 
P- 
P 
c4 
CD 
oo 
o 
O - GO 
o PS 
p 
CD P 
P'S" 
i-i 
r+- 
CD 2. 
e4 M 
CD ks 
w P 
el 
I 
ot 
Oo 
3 g. 
cd 
o g; 
’-'•S 
►dEt 
o sr 
<rt- CD 
p q. 
CD 
o 
td 
t —•• 
O 
tr 
o 
i 
*12 
g B 
p P r 
S 
CD 
P- 
*—<• 
e 
3 
g 
CD 
CL 
It is seen here that germination was more in ordinary distilled water 
than in the supposedly non-toxic water (U. S. Bureau of Soils) 
and that still higher germination was attained in tap water and 
higher yet in the nutrient lettuce broth. Copper sulphate JST/800 is 
fatal to all of the spores and only 10 per cent, germination was found 
in the JST/1600. The per cent, of germination increased almost in 
proportion to the weakening of the solution beyond this point. In 
the case of the Bordeaux filtrate it is -seen that the first filtrate was 
fatal to all the spores while the second did not prevent germination 
at all. 
To test the longevity of ascospores, collections were made on cover 
glasses and placed away in sterile petri dishes. These were tested in 
duplicate or triplicate the first and second day and every seventh 
day thereafter until no germination was apparent, using either lettuce 
broth or 4 per cent, sugar solution as the medium. The results of 
these tests appear below: 
TABLE XI.—SHOWING LONGEVITY OF ASCOSPORES. 
Age in Days. 
1 
2 
7 
14 
21 
28 
35 
42 
49 
56 
63 
70 
77 
84 
91 
Per Cent of 
Germination. 
100 
100 
25 
50 
50 
4 
65 
10 
50 
50 
90 
75 
95 
90 
90 
50 
Medium 
Used in 
Cultures. 
pi 
+2 
o 
u 
pq 
pi 
o 
(h 
m 
pi 
+= 
o 
(H 
pq 
pi 
4-3 
O 
t-i 
m 
pi 
o 
l-c 
pq 
pi 
+3 
o 
pq 
pi 
+= 
o 
pq 
4-3 
O 
PQ 
c3 
si 
Jh 
pi 
4-3 
O 
u 
pq 
pi 
o 
pq 
pi 
-u 
O 
u 
pq 
d 
o 
• r4 
43 
_P 
"o 
CO 
u 
aS 
0 
<15 
0 
0 
0 
0 
0 
0 
M 
M 
bfl 
0 
0 
0 
60 
o 
p 
4-3 
a 
P 
43 
O 
P 
4-3 
0 
P 
0 
P 
0 
P 
4-3 
0 
P 
4-3 
0 
P 
4-3 
P 
m 
P 
m 
P 
CO 
0 
P 
4-3 
0 
5 
0 
P 
43 
P 
m 
43 
3 
43 
>3 
43 
0 
3 
3 
4-3 
3 
+3 
3 
4-3 
3 
4-3 
3 
4-3 
3 
43 
3 
4-> 
3 
