The Bulletin 
23 
were obtained, of which, at the present writing, only 6 are alive. In 
1915 two of these vines bloomed and each produced only staminate 
flowers. 
In 1915, 47 seedlings were obtained from the same mother vine, and 
in 1916, 530 more seedlings from a similar origin but from different 
vines, were set in the nursery row for observation and study. 
In the fall of 1913 a large number of seeds were taken from fruits 
of the Hope vine which had developed under natural conditions, that 
is, with the flowers uncovered and subject to any cross-pollination that 
might take place. From these seeds 142 plants were obtained and some 
of these have bloomed this year (1916). The types and numbers of 
vines that have bloomed and been observed are as follows: staminate 
vines, 21; hermaphrodite vines with upright stamens, 31; hermaphrodite 
vines with reflexed stamens, 4. The number of vines not blooming was 
86. Of these 86 non-blooming vines, it was confidently expected by the 
writer that 17 vines, by virtue of their size and age, would produce 
hermaphrodite flowers with reflexed stamens. The remaining 59 seed¬ 
lings were too small and weak to produce any flowers. If we accept the 
numbers of these vines as stated, the ratios of the different types is 
21:31:21. What the final ratio will be when all of the vines shall have 
bloomed becomes, at most, an interesting matter for speculation. 
TABLE No. 5 
The Extent of Natural Crossing That May Take Place Among the Self-fertile Seedling 
Vines of the Hope Vine Progeny When Growing in Mass 
Lot 
Color Mother 
Vines 
Total 
Number 
Seedlings 
Grown 
Number 
Seedlings 
Light 
Color 
N umber 
Seedlings 
Dark 
Color 
1_ 
Light_ 
112 
110 
2 
2_ 
Light_ 
64 
58 
6 
3_ 
Light_ 
59 
55 
4 
4_ 
Light_ 
16 
13 
3 
5_ 
Light_ 
105 
88 
17 
6_ 
Light_ 
20 
19 
1 
7 
Light_ 
104 
104 
0 
8_ 
Light_ 
40 
38 
2 
9_ 
Light _ 
96 
90 
6 
Totals_ _ - - - 
616 
575 
41 
In this work, seeds from light-colored vines only were selected, be¬ 
cause, as the factor for coloration is dominant to its absence, the appear¬ 
ance of dark-colored seedlings in any of these several lots would be 
strong evidence in favor of cross-pollination. 
From the evidence presented in Table Ho. 5, we learn that in eight out 
of nine lots of seedlings some dark-colored plants have appeared. By 
way of explanation, it should be said that the dark-colored vines, 547 
in number, standing adjacent to the light-colored ones from which the 
