24 
The Bulletin 
seeds were taken, were Heterozygous for the light and the dark colors. 
If, then, any crossing took place between the light and the dark vines, the 
resultant dark-colored seedlings, according to the Mendelian laws, repre¬ 
sent only about one-half of the actual number of such a cross. This 
very meager evidence gives us some idea of the actual amount of inter¬ 
crossing that takes place among our strain of hermaphrodite vines that 
have the upright stamen. The ratios of colors that were obtained 
among these seedlings, however, are not to be taken as an index of the 
extent of cross-pollination that usually takes place among such seedling 
vines, but simply as an indication that some crossing has resulted. 
The fourth point under consideration deals with the ratios between 
types of flowers among seedling vines when both of the parents bear 
reflexed stamens. Since Bui. INTo. 209 of this Station (1) discusses at 
some length the self-sterility and the inter-sterility of all rotundifolia 
grape vines which bear reflexed type of stamen, we understand imme¬ 
diately why such seedling vines have not been produced and, hence, why 
we cannot establish a ratio between types of flowers resulting from such 
a cross. 
The fifth point under consideration deals with the ratios between 
types of flowers in the progeny when hermaphrodite flowers with up¬ 
right stamens are crossed with staminate flowers. In 1915 twenty flower 
clusters of this Hope vine were carefully emasculated and bagged and 
later, after about, twenty-four hours, viable pollen from a staminate vine 
was brushed over the pistils. Ho fruits developed in any of these bags. 
The same spring 53 clusters on one of its self-fertile seedling vines 
were carefully emasculated and, when the pistils became receptive, were 
pollinated with fresh, viable pollen from a staminate vine. ISTot one of 
these flower clusters, however, has produced so much as a berry. At 
the same time, two large flower clusters on this same self-fertile vine, 
the flowers of which were not emasculated, were dusted with viable 
pollen from a staminate vine which is known to be homozygous for dark 
color. Eighteen berries ripened and a total of 61 seeds were obtained. 
These seeds were planted in 1916 and from them 59 seedlings were 
grown. Each of these 59 seedlings were light in color and not one was 
dark. This indicates that the seedlings were not the result of a cross 
between two separate vines, but the product from self-fertilized flowers 
on the mother vine, since dark color of vine is dominant over light 
color. [Beimer and Detjen (5), Hedrick and Anthony (3)]. 
In 1916 flowers of twelve more clusters from light-colored self-fertile 
seedlings were carefully emasculated and later hand pollinated with 
viable pollen from another staminate vine which is also known to be 
homozygous for dark color. Hot one of these flower clusters produced 
a berry. At the same time the flowers on 50 or more clusters on similar 
light-colored, self-fertile seedling vines (all seedlings of Scuppernong 
and Hope) were emasculated, although less carefully in regard to the 
selling of the flowers, and immediately cross-pollinated with pollen from 
a staminate vine which was known to be homozygous for dark color. 
