The Bulletin 
29 
of trembles bad developed by July 16, and deatli occurred two days 
later. Food was refused during these two days, and there was a de¬ 
crease in weight from 102 to 77 pounds during the 29 days of inter¬ 
mittent feeding. 
Ewe 26 was given a ration of snakeroot and grain for 22 days, be¬ 
tween June 17 and August 2. Ho symptoms of trembles developed 
during this period. The initial weight of this animal was 91 pounds 
and the weight at the time the experiment was discontinued was 74 
pounds. 
The control ewe (Ho. 10) was maintained on pasture alone from 
June 17 to July 28. On July 28 she was put ki a pen and was given 
a ration of white snakeroot and grain until her death, which occurred 
on August 2. This ewe trembled only slightly, was very weak and 
emaciated, and lost 20 pounds during the experiment. Although the 
symptoms were not as marked in this case as in ewe 11, yet all condi¬ 
tions indicated that death was due to trembles. 
Experiment 2 .—Three ewes, Hos. 14, 23, and 26, were employed in 
Experiment 2. This experiment was planned to confirm the results 
secured in Experiment 1. Since the animals used in Experiment 1 had 
refused to eat any considerable quantity of snakeroot when it was fed 
separately, it was decided to pass the weed through an ensilage cutter 
and mix it with an equal quantity of weight of grain. One pound of 
this mixed feed was given each animal twice daily. 
On the sixth day after ewe 14 was placed on the experiment she had 
developed trembles and died on the following day. Her initial weight 
was 80 pounds and her weight at death was 73 pounds. 
The first symptom of trembles in the case of ewe 23 was noted 19 
days after the experiment was begun. A well-defined case of trembles 
developed in this animal, and she died six days after the first symptoms 
were noticed. Her weight when feeding was begun was 70 pounds, and 
there was a loss in weight of 8 pounds during the 25 days. 
Since ewe 26 had shown no ill effects from the feeding of white snake¬ 
root in Experiment 1, she was used in this experiment. It will be 
recalled that the weed was fed separately and was not ground in the 
first experiment. Ewe 26 had eaten only sparingly of the weed in this 
experiment. However, after 16 days feeding with the mixed ration, a 
very typical case of trembles developed. The feeding of the weed was 
therefore discontinued and she was put on pasture. 
Experiment 3 .—In this experiment, ewes 12, 7, 29, 27, and 19 were 
fed the mixed ration to determine the amount of weed and the length 
of time required to develop trembles. Table 1 shows clearly the varia¬ 
tion that exists with reference to these two points. 
