36 
The Bulletin 
until July 5. They ate ravenously, however, and by July 8 had eaten 
the water soluble material from 1800 grams of dry white snakeroot 
with no ill effect except the disgestive disturbance mentioned. For the 
next fourteen days they were given an aqueous extract prepared from the 
fresh weed. At the end of this time, they were in good condition and the 
feeding was discontinued. It does not appear from this that the active 
principle is water soluble, or if it is, the heat of desiccation destroys 
its toxicity. It will be recalled in this connection, that the feeding of 
aqueous extracts previously reported by Crawford (1908) were not fol¬ 
lowed by trembles. 
Experiment 16 .—The feeding of three guinea pigs with material ex¬ 
tracted with dilute hydrochloric acid was begun on June 28. This ex¬ 
tract was made neutral with sodium hydroxid with the consequent pre¬ 
cipitation of salt. 1STo. 1 was sick on July 2, was very inactive on July 
3, hut ate of the grain and clover. He refused to eat on the morning 
of the following day, and died at noon. The other two were still un¬ 
affected on July 8 when feeding was discontinued. 
Experiment 17 .—The feeding of alcohol soluble products was begun 
on the evening of July 3. By the evening of July 5, two guinea pigs 
had eaten the extract from 1,800 grams of dry weed. Ho ill effects 
whatsoever followed during the succeeding two weeks in which they 
were kept under observation. 
i£q U 0 atS 9.I0AV s§id uauinS xis ^uouiLiedxa siijq up —'pi yudmuddxtf 
means of a pipette the expressed sap or juice of white snakeroot. On 
the evening of July 20, two large animals, each weighing nearly 800 
grams, were given 10 to 12 c.c. apiece. On the following morning both 
were noted to be droopy and refused to eat. Ho. 1 was so weak that 
he could scarcely walk and seemed unable to control the movements in 
the hind limbs. Each was given about 12 c.c. on the morning of the 
21st and an equal quantity at noon. At 5 P. M. Ho. 1 died, having 
been given a total of about 35 c.c. Ho. 2 was given two feedings on the 
22d and had received a total of about 50 c.c. during the three days of 
feeding. He became more inactive and listless daily, passed into a 
state of coma and died July 26. 
On the morning of the 24th two more guinea pigs were placed in 
this cage and each was given in three feedings about 25 c.c. Both were 
sick on the following morning, hut were given during the day three 
quantities of expressed juice totaling about 25 c.c. for each animal. 
On the following morning Ho. 3 died. Ho 4 was fed three times daily 
on the 26th, 27th, and 28th. Feeding was discontinued on this last date 
and he died three days later. 
