The Bulletin 
53 
A manifest stiffness of the limbs was apparent on June 11. On June 13, she 
refused grain and ate only a small amount of kale. She appeared stiff, droopy, 
and dull. In the afternoon, respirations were slightly jerky. On the morning 
of June 14, she trembled slightly and was prone to lie down at all times. When 
made to stand she would tremble, and the longer she was kept on her feet the 
more intense the trembling. In the afternoon the symptoms were more aggra¬ 
vated. She trembled in all parts of the body and after a paroxysm of trem¬ 
bling would fall down apparently exhausted. At intervals she gritted her 
teeth. On the 15th, while lying down, she attempted to eat kale. Trembling 
became more aggravated, as did the gritting of teeth, and she appeared weaker 
than on the previous day. She did not eat during the afternoon. On the morn¬ 
ing of June 17 she was found prostrate on her side. Respiration was labored, 
eyes were set, and legs were intermittently trembling. She died about 12 :30 
P. M. 
Blood Study. On May 18 a study was made of the blood of lamb 244 to 
determine the normal condition, with the following results: 
Numerical count: 
Erythrocytes .... 13,560,000 
Leucocytes .. 12,000 
Differential count: 
Neutrophiles . 20 per cent 
Lymphocytes, large . 7 per cent 
Lymphocytes, small . 71 per cent 
Eosinopliiles . 1 per cent 
Mast cells . 1 per cent 
Hemaglobin . 75 per cent 
On June 16, after the symptoms of milksickness had developed, another 
study was made. The results are as follows: 
Numerical count: 
Erythrocytes . 15,600,000 
Leucocytes . 10,000 
Differential count: 
Neutrophiles . 44 per cent 
Lymphocytes, large . 6 per cent 
Lymphocytes, small .. 49 per cent 
Mast cells . 1 per cent 
Hemaglobin . 75 per cent 
There was an increase of neutrophiles at the expense of the lymphocytes 
and an apparent increase in the erythrocytes. The lamb had refused water 
for at least forty-eight hours before this second study was made, which may 
account for the apparent increase. The eosinophiles had entirely disappeared. 
The hemaglobin remained the same. 
Urine Study. On June 17, when the autopsy was made, the urine was 
forced out of the normal outlet by gentle pressure upon the bladder and was 
thus kept free from albuminous material. The following is the result of the 
analysis: 
Physical examination: 
Sediment—Present. 
Transparency—Cloudy. 
Specific gravity—Insufficient amount for test. 
