56 
The Bulletin 
Clinical Notes. On July 17 ewe 12 appeared droopy, dull, and stood with 
eyes half-closed. On the 19tli breathing was jerky and trembling was noted 
in both fore and hind limbs. After she had lain quiet for a while she woulu 
stand for several minutes, and with the onset of trembling would lie down 
again. Her appetite was good. On July 21 the symptoms were more aggra¬ 
vated. She would stand with all four feet close together under the body, 
with back arched, and was quite stiff. On the 22d, trembling was more vio¬ 
lent, but her appetite remained good. In the afternoon, she passed into a 
comatose condition. There was little appreciable change on July 23, but 
the symptoms were more aggravated. On July 24 she was killed and an au¬ 
topsy was performed. 
It should be noted with reference to this ewe that she had recovered from 
trembles from feeding experiments of the previous year. This indicates that 
one attack does not render immunity to a subsequent attack, a fact in keeping 
with popular belief. 
Blood Study. On May 18 a study was made of the blood of ewe 12 to 
determine its normal condition. The following is the result: 
Numerical count: 
Erythrocytes . 8,240,000 
Leucocytes . 12,000 
Differential count: 
Neutrophiles . 47 per cent 
Lymphocytes, large . 7 per cent 
Lymphocytes, small . 40 per cent 
Eosinopliiles . 3 per cent 
Mast cells . None 
Hemaglobin . 70 per cent 
On July 22, after symptoms of trembles had become well developed, another 
blood study was made with the following result: 
Numerical count: 
Erythrocytes . 8,320,000 
Leucocytes . 5.000 
Differential count: 
Neutrophiles . 66 per cent 
Lymphocytes, large . 8 per cent 
Lymphocytes, small . 22 per cent 
Eosinophiles ... None 
Mast cells ... 4 per cent 
Hemaglobin ... 75 per cent 
There was an increase of neutrophiles at the expense of the small lympho¬ 
cytes. There was a disappearance of the eosinophiles and the appearance of 
mast cells. The hemaglobin remained practically the same and there was 
no change in the number of erythrocytes. There was a considerable decrease 
in the leucocytes. 
Urine Study. An analysis of the urine was made on June 18, with the- 
following results: 
Physical examination: 
Transparency—Pale straw color. 
Specific gravity—1014. 
