Limits in Hybridization 
15 
of the seedbed, was secured. This hybrid vine is living today and will 
be fully discussed in our succeeding bulletin. This is the first Herbe- 
mout-Rotundifolia hybrid that was ever produced and recorded. 
In 1916 and 1917 Herbemont was again crossed with V. rotundifolia, 
G-52 being the male parent. Five flower clusters on the Herbemont 
vine were prepared in 1916 and later cross-pollinated with pollen from 
G-52. A total of 35 seeds was harvested and from this lot of seed 7 
fairly strong hybrid vines were secured. Seven flower clusters on the 
Herbemont vine were prepared in 1917 and again cross-pollinated with 
pollen from G-52. A total of 86 seeds was harvested from this cross but 
only 5 fairly strong hybrid vines were secured. Of these two lots of 
hybrid vines only seven vines are at present (1919) alive. Most of the 
hybrids of Herbemont with Y. rotundifolia are constitutionally weak 
and only a comparatively few seem able to survive. 
Vitis Rotundifolia X Vitis Bourquianiana 
The results of the reciprocal cross Y. rotundifolia x Y. bourquiniana 
var. Herbemont have been altogether negative thus far. In 1916 two 
Rotundifolia seedlings, X-32 and a perfect flowered selfed seedling of 
Hope, were used. Ten bags covering 20 flower clusters were applied 
to the vine X-32 and four bags covering as many flower clusters were 
applied to the perfect flowered seedling of Hope. The expanding flowers 
were in due season dusted with fresh pollen from the variety Herbe¬ 
mont. Xo fruits were secured from either of these pollinations. 
In 1917 three flower clusters on a Scuppernong vine 5 " which had been 
forced in the greenhouse were bagged and cross-pollinated with Herbe¬ 
mont pollen. During the same season seven flower clusters on a Scup¬ 
pernong vine in the vineyard were bagged and cross-pollinated with 
Herbemont pollen. X-28 and 0-23, two seedlings of Scuppernong x 
Dark Male vine Xo. 1 had one flower cluster each bagged and crossed 
with Herbemont pollen. E-19, a seedling of Thomas x Light Male vine 
Xo. 2 also had two flower clusters treated in the same manner. Xot one 
berry was produced in all of these crosses. 
Rotundifolia-Munsoniana Hybrid\ X Vitis Bourquiniana 
In 1917 three flower clusters of the variety Eden, a Rotundifolia- 
Munsoniana hybrid, were bagged and cross-pollinated with Herbemont 
pollen. Xo fruit was secured from this cross. 
* This Scuppernong vine was grown in a box and some time during the month of 
February was placed in the greenhouse for forcing. In order to ascertain whether this 
forcing might exert any evil influences in regard to the production of fruit, one flower 
cluster was cross-pollinated with normal Rotundifolia pollen and typical fruit was ob¬ 
tained. The vine, therefore, was in proper condition to enter into the experimental 
work. 
